Imperial College London


Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS



+44 (0)20 7594 7706paul.french Website




Ms Judith Baylis +44 (0)20 7594 7713




609Blackett LaboratorySouth Kensington Campus






BibTex format

author = {Sherlock, B and Warren, SC and Alexandrov, Y and Yu, F and Stone, J and Knight, J and Neil, MAA and Paterson, C and French, PMW and Dunsby, CW},
doi = {10.1002/jbio.201700131},
journal = {Journal of Biophotonics},
title = {In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation},
url = {},
volume = {11},
year = {2017}

RIS format (EndNote, RefMan)

AB - This paper reports a handheld multiphoton fluorescence microscope designed for clinical imaging that incorporates axial motion compensation and lateral image stabilization. Spectral domain optical coherence tomography is employed to track the axial position of the skin surface, and lateral motion compensation is realised by imaging the speckle pattern arising from the optical coherence tomography beam illuminating the sample. Our system is able to correct lateral sample velocities of up to ~65 μm s-1. Combined with the use of negative curvature microstructured optical fibre to deliver tunable ultrafast radiation to the handheld multiphoton scanner without the need of a dispersion compensation unit, this instrument has potential for a range of clinical applications. The system is used to compensate for both lateral and axial motion of the sample when imaging human skin in vivo.
AU - Sherlock,B
AU - Warren,SC
AU - Alexandrov,Y
AU - Yu,F
AU - Stone,J
AU - Knight,J
AU - Neil,MAA
AU - Paterson,C
AU - French,PMW
AU - Dunsby,CW
DO - 10.1002/jbio.201700131
PY - 2017///
SN - 1864-063X
TI - In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation
T2 - Journal of Biophotonics
UR -
UR -
VL - 11
ER -