Imperial College London

ProfessorPaulFrench

Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

478 results found

Munro I, Garcia E, Yan M, Guldbrand S, Kumar S, Kwakwa K, Dunsby C, Neil MAA, French PMWet al., 2019, Accelerating single molecule localization microscopy through parallel processing on a high-performance computing cluster, JOURNAL OF MICROSCOPY, Vol: 273, Pages: 148-160, ISSN: 0022-2720

JOURNAL ARTICLE

Guo W, Kumar S, Görlitz F, Garcia E, Alexandrov Y, Munro I, Kelly DJ, Warren S, Thorpe P, Dunsby C, French Pet al., 2019, Automated Fluorescence Lifetime Imaging High-Content Analysis of Förster Resonance Energy Transfer between Endogenously Labeled Kinetochore Proteins in Live Budding Yeast Cells., SLAS Technol

We describe an open-source automated multiwell plate fluorescence lifetime imaging (FLIM) methodology to read out Förster resonance energy transfer (FRET) between fluorescent proteins (FPs) labeling endogenous kinetochore proteins (KPs) in live budding yeast cells. The low copy number of many KPs and their small spatial extent present significant challenges for the quantification of donor fluorescence lifetime in the presence of significant cellular autofluorescence and photobleaching. Automated FLIM data acquisition was controlled by µManager and incorporated wide-field time-gated imaging with optical sectioning to reduce background fluorescence. For data analysis, we used custom MATLAB-based software tools to perform kinetochore foci segmentation and local cellular background subtraction and fitted the fluorescence lifetime data using the open-source FLIMfit software. We validated the methodology using endogenous KPs labeled with mTurquoise2 FP and/or yellow FP and measured the donor fluorescence lifetimes for foci comprising 32 kinetochores with KP copy numbers as low as ~2 per kinetochore under an average labeling efficiency of 50%. We observed changes of median donor lifetime ≥250 ps for KPs known to form dimers. Thus, this FLIM high-content analysis platform enables the screening of relatively low-copy-number endogenous protein-protein interactions at spatially confined macromolecular complexes.

JOURNAL ARTICLE

Davis SPX, Wisniewski L, Kumar S, Correia T, Arridge SR, Frankel P, McGinty J, French PMWet al., 2018, Slice-illuminated optical projection tomography, OPTICS LETTERS, Vol: 43, Pages: 5555-5558, ISSN: 0146-9592

JOURNAL ARTICLE

Gorlitz F, Guldbrand S, Runcorn TH, Murray RT, Jaso-Tamame AL, Sinclair HG, Martinez-Perez E, Taylor JR, Neil MAA, Dunsby C, French PMWet al., 2018, easySLM-STED: Stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning, JOURNAL OF BIOPHOTONICS, Vol: 11, ISSN: 1864-063X

JOURNAL ARTICLE

Lagarto JL, Dyer BT, Talbot CB, Peters NS, French PMW, Lyon AR, Dunsby Cet al., 2018, Characterization of NAD(P)H and FAD autofluorescence signatures in a Langendorff isolated-perfused rat heart model, BIOMEDICAL OPTICS EXPRESS, Vol: 9, Pages: 4961-4978, ISSN: 2156-7085

JOURNAL ARTICLE

Sparks H, Kondo H, Hooper S, Munro I, Kennedy G, Dunsby C, French P, Sahai Eet al., 2018, Heterogeneity in tumor chromatin-doxorubicin binding revealed by in vivo fluorescence lifetime imaging confocal endomicroscopy, NATURE COMMUNICATIONS, Vol: 9, ISSN: 2041-1723

JOURNAL ARTICLE

Wisniewski L, Davis S, Lockwood N, Mcginty J, French P, Frankel Pet al., 2018, A role for p130Cas in venous sprouting and lymphangiogenesis in the zebrafish, 5th Congress of the ESC-Council-on-Basic-Cardiovascular-Science on Frontiers in Cardio Vascular Biology, Publisher: OXFORD UNIV PRESS, Pages: S90-S90, ISSN: 0008-6363

CONFERENCE PAPER

Rutter GA, Haythorne EA, Georgiadou E, Xavier GDS, Pullen TJ, Rizzuto R, Martinez-Sanchez A, McGinty JA, French PMet al., 2018, Pancreatic beta cell-selective deletion of the mitochondrial calcium uniporter (MCU) impairs glucose-stimulated insulin secretion in vitro but not in vivo, Publisher: WILEY, Pages: 42-42, ISSN: 0742-3071

CONFERENCE PAPER

Kim Y, Warren S, Favero F, Stone J, Clegg J, Neil M, Paterson C, Knight J, French P, Dunsby Cet al., 2018, Semi-random multicore fibre design for adaptive multiphoton endoscopy, OPTICS EXPRESS, Vol: 26, Pages: 3661-3673, ISSN: 1094-4087

JOURNAL ARTICLE

Sherlock B, Warren SC, Alexandrov Y, Yu F, Stone J, Knight J, Neil MAA, Paterson C, French PMW, Dunsby Cet al., 2018, In vivo multiphoton microscopy using a handheld scanner with lateral and axial motion compensation, JOURNAL OF BIOPHOTONICS, Vol: 11, ISSN: 1864-063X

JOURNAL ARTICLE

Chen L, Li G, Li Y, Li Y, Zhu H, Tang L, French P, McGinty J, Ruan Set al., 2017, UbasM: An effective balanced optical clearing method for intact biomedical imaging, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322

JOURNAL ARTICLE

Lagarto J, Hares JD, Dunsby C, French PMWet al., 2017, Development of Low-Cost Instrumentation for Single Point Autofluorescence Lifetime Measurements, JOURNAL OF FLUORESCENCE, Vol: 27, Pages: 1643-1654, ISSN: 1053-0509

JOURNAL ARTICLE

Gorlitz F, Corcoran DS, Castano EAG, Leitinger B, Neil MAA, Dunsby C, French PMWet al., 2017, Mapping Molecular Function to Biological Nanostructure: Combining Structured Illumination Microscopy with Fluorescence Lifetime Imaging (SIM, PHOTONICS, Vol: 4, ISSN: 2304-6732

JOURNAL ARTICLE

Noble E, Kumar S, Gorlitz FG, Stain C, Dunsby C, French PMWet al., 2017, In vivo label-free mapping of the effect of a photosystem II inhibiting herbicide in plants using chlorophyll fluorescence lifetime, PLANT METHODS, Vol: 13, ISSN: 1746-4811

JOURNAL ARTICLE

Van de Pette M, Abbas A, Feytout A, McNamara G, Bruno L, To WK, Dimond A, Sardini A, Webster Z, McGinty J, Paul EJ, Ungless MA, French PMW, Withers DJ, Uren A, Ferguson-Smith AC, Merkenschlager M, John RM, Fisher AGet al., 2017, Visualizing Changes in Cdkn1c Expression Links Early-Life Adversity to Imprint Mis-regulation in Adults, CELL REPORTS, Vol: 18, Pages: 1090-1099, ISSN: 2211-1247

JOURNAL ARTICLE

Perdios L, Lowe AR, Saladino G, Bunney TD, Thiyagarajan N, Alexandrov Y, Dunsby C, French PMW, Chin JW, Gervasio FL, Tate EW, Katan Met al., 2017, Conformational transition of FGFR kinase activation revealed by site-specific unnatural amino acid reporter and single molecule FRET, SCIENTIFIC REPORTS, Vol: 7, ISSN: 2045-2322

JOURNAL ARTICLE

Go¨rlitz F, Corcoran DS, Sparks H, Leitinger B, Neil M, Dunsby C, French Pet al., 2017, Mapping molecular function to biological nanostructure: Combining structured illumination microscopy with fluorescence lifetime imaging

© 2017 OSA. We report the enhancement of spatial resolution and sensitivity of wide-field time-gated imaging and the combination with SIM to map molecular function using FRET to biological nanostructure below the conventional diffraction limit.

CONFERENCE PAPER

Gorlitz F, Kelly DJ, Warren SC, Alibhai D, West L, Kumar S, Alexandrov Y, Munro I, Garcia E, McGinty J, Talbot C, Serwa RA, Thinon E, da Paola V, Murray EJ, Stuhmeier F, Neil MAA, Tate EW, Dunsby C, French PMWet al., 2017, Open Source High Content Analysis Utilizing Automated Fluorescence Lifetime Imaging Microscopy, JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, ISSN: 1940-087X

JOURNAL ARTICLE

Andrews N, Davis S, Hay C, Kumar S, Ramel M-C, Bugeon L, McGinty J, Dallman MJ, French PMWet al., 2017, Functional imaging of live Zebrafish using fluorescence lifetime optical projection tomography, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Sparks H, Gorlitz F, Kelly DJ, Warren SC, Kellett PA, Garcia E, Dymoke-Bradshaw AKL, Hares JD, Neil MAA, Dunsby C, French PMWet al., 2017, Characterisation of new gated optical image intensifiers for fluorescence lifetime imaging, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 88, ISSN: 0034-6748

JOURNAL ARTICLE

Cortes E, Huidobro PA, Sinclair HG, Guldbrand S, Peveler WJ, Davies T, Parrinello S, Gorlitz F, Dunsby C, Neil MAA, Sivan Y, Parkin IP, French PMW, Maier SAet al., 2016, Plasmonic Nanoprobes for Stimulated Emission Depletion Nanoscopy, ACS NANO, Vol: 10, Pages: 10454-10461, ISSN: 1936-0851

JOURNAL ARTICLE

Margineanu A, Chan JJ, Kelly DJ, Warren SC, Flatters D, Kumar S, Katan M, Dunsby CW, French PMWet al., 2016, Screening for protein-protein interactions using Forster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) (vol 6, 28186, 2016), SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

Warren SC, Kim Y, Stone JM, Mitchell C, Knight JC, Neil MAA, Paterson C, French PMW, Dunsby Cet al., 2016, Adaptive multiphoton endomicroscopy through a dynamically deformed multicore optical fiber using proximal detection, OPTICS EXPRESS, Vol: 24, Pages: 21474-21484, ISSN: 1094-4087

JOURNAL ARTICLE

Semplici F, Mondragon A, Macintyre B, Madeyski-Bengston K, Persson-Kry A, Barr S, Ramne A, Marley A, McGinty J, French P, Soedling H, Yokosuka R, Gaitan J, Lang J, Migrenne-Li S, Philippe E, Herrera PL, Magnan C, Xavier GDS, Rutter GAet al., 2016, Cell type-specific deletion in mice reveals roles for PAS kinase in insulin and glucagon production, DIABETOLOGIA, Vol: 59, Pages: 1938-1947, ISSN: 0012-186X

JOURNAL ARTICLE

Kwakwa K, Savell A, Davies T, Munro I, Parrinello S, Purbhoo MA, Dunsby C, Neil MAA, French PMWet al., 2016, easySTORM: a robust, lower-cost approach to localisation and TIRF microscopy, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 948-957, ISSN: 1864-063X

JOURNAL ARTICLE

Chennell G, Willows RJW, Warren SC, Carling D, French PMW, Dunsby C, Sardini Aet al., 2016, Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM, SENSORS, Vol: 16, ISSN: 1424-8220

JOURNAL ARTICLE

Kumar S, Lockwood N, Ramel M-C, Correia T, Ellis M, Alexandrov Y, Andrews N, Patel R, Bugeon L, Dallman MJ, Brandner S, Arridge S, Katan M, McGinty J, Frankel P, French PMWet al., 2016, Quantitative in vivo optical tomography of cancer progression & vasculature development in adult zebrafish, ONCOTARGET, Vol: 7, Pages: 43939-43948, ISSN: 1949-2553

JOURNAL ARTICLE

Sherlock B, Yu F, Stone J, Warren S, Paterson C, Neil MAA, French PMW, Knight J, Dunsby Cet al., 2016, Tunable fibre-coupled multiphoton microscopy with a negative curvature fibre, JOURNAL OF BIOPHOTONICS, Vol: 9, Pages: 715-720, ISSN: 1864-063X

JOURNAL ARTICLE

Margineanu A, Chan JJ, Kelly DJ, Warren SC, Flatters D, Kumar S, Katan M, Dunsby CW, French PMWet al., 2016, Screening for protein-protein interactions using Forster resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM), SCIENTIFIC REPORTS, Vol: 6, ISSN: 2045-2322

JOURNAL ARTICLE

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