Imperial College London
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ProfessorPaulFrench

Faculty of Natural Sciences, Department of Physics

Professor of Physics
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Van:2022:10.1038/s41467-022-30022-2,
author = {Van, de Pette M and Dimond, A and Galvao, AM and Millership, SJ and To, W and Prodani, C and McNamara, G and Bruno, L and Sardini, A and Webster, Z and McGinty, J and French, PMW and Uren, AG and Castillo-Fernandez, J and Watkinson, W and Ferguson-Smith, AC and Merkenschlager, M and John, RM and Kelsey, G and Fisher, A},
doi = {10.1038/s41467-022-30022-2},
journal = {Nature Communications},
title = {Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice},
url = {http://dx.doi.org/10.1038/s41467-022-30022-2},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Transmission of epigenetic information between generations occurs in nematodes, flies and plants, mediated by specialised small RNA pathways, modified histones and DNA methylation. Similar processes in mammals can also affect phenotype through intergenerational or trans-generational mechanisms. Here we generate a luciferase knock-in reporter mouse for the imprinted Dlk1 locus to visualise and track epigenetic fidelity across generations. Exposure to high-fat diet in pregnancy provokes sustained re-expression of the normally silent maternal Dlk1 in offspring (loss of imprinting) and increased DNA methylation at the somatic differentially methylated region (sDMR). In the next generation heterogeneous Dlk1 mis-expression is seen exclusively among animals born to F1-exposed females. Oocytes from these females show altered gene and microRNA expression without changes in DNA methylation, and correct imprinting is restored in subsequent generations. Our results illustrate how diet impacts the foetal epigenome, disturbing canonical and non-canonical imprinting mechanisms to modulate the properties of successive generations of offspring.
AU  - Van,de Pette M
AU  - Dimond,A
AU  - Galvao,AM
AU  - Millership,SJ
AU  - To,W
AU  - Prodani,C
AU  - McNamara,G
AU  - Bruno,L
AU  - Sardini,A
AU  - Webster,Z
AU  - McGinty,J
AU  - French,PMW
AU  - Uren,AG
AU  - Castillo-Fernandez,J
AU  - Watkinson,W
AU  - Ferguson-Smith,AC
AU  - Merkenschlager,M
AU  - John,RM
AU  - Kelsey,G
AU  - Fisher,A
DO  - 10.1038/s41467-022-30022-2
PY  - 2022///
SN  - 2041-1723
TI  - Epigenetic changes induced by in utero dietary challenge result in phenotypic variability in successive generations of mice
T2  - Nature Communications
UR  - http://dx.doi.org/10.1038/s41467-022-30022-2
UR  - http://hdl.handle.net/10044/1/97090
VL  - 13
ER  -
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