Epigenetic reprogramming encompasses changes in nuclear architecture and epigenetic modifications, eventually leading to a shift in gene expression profile. At the molecular level, such a process is connected with the erasure of epigenetic marks including DNA methylation and histone modifications. Our laboratory uses in vivo models to elucidate molecular mechanisms underlying naturally occurring reprogramming events. The knowledge gained allows us to design in vitro experimental systems and to use biochemical approaches to investigate further molecular details.
We study the reprogramming events both in the developing mouse germ line and in the mouse zygote. The former involves both genome-wide DNA demethylation and chromatin remodelling, whereas reprogramming the zygote involves genome-wide DNA demethylation affecting only the paternal genome a few hours after fertilisation.
As epigenetic reprogramming plays a pivotal role in the dedifferentiation and the reversal of cell fate decisions, investigation of molecular pathways underlying such processes provides direct mechanistic links to regeneration and cancer.
et al., 2023, Quantifying propagation of DNA methylation and hydroxymethylation with iDEMS., Nat Cell Biol, Vol:25, Pages:183-193
et al., 2021, Sex-specific chromatin remodelling safeguards transcription in germ cells, Nature, Vol:600, ISSN:0028-0836, Pages:737-742
et al., 2021, Specification and epigenomic resetting of the pig germline exhibit conservation with the human lineage, Cell Reports, Vol:34, ISSN:2211-1247
et al., 2020, TBPL2/TFIIA complex establishes the maternal transcriptome through oocyte-specific promoter usage, Nature Communications, Vol:11, ISSN:2041-1723
et al., 2020, Global regulatory transitions at core promoters demarcate the mammalian germline cycle