Imperial College London

Dr Philippa C. May

Faculty of MedicineDepartment of Immunology and Inflammation

Visiting Researcher
 
 
 
//

Contact

 

philippa.may

 
 
//

Location

 

4N5Commonwealth BuildingHammersmith Campus

//

Summary

 

Publications

Publication Type
Year
to

28 results found

Rowan AG, May P, Badhan A, Herrera C, Watber P, Penn R, Crone MA, Storch M, Garson JA, McClure M, Freemont PS, Madona P, Randell P, Taylor GPet al., 2021, Optimized protocol for a quantitative SARS-CoV-2 duplex RT-qPCR assay with internal human sample sufficiency control., Journal of Virological Methods, Vol: 294, Pages: 1-7, ISSN: 0166-0934

There is growing evidence that measurement of SARS-CoV-2 viral copy number can inform clinical and public health management of SARS-CoV-2 carriers and COVID-19 patients. Here we show that quantification of SARS-CoV-2 is feasible in a clinical setting, using a duplex RT-qPCR assay which targets both the E gene (Charité assay) and a human RNA transcript, RNase P (CDC assay) as an internal sample sufficiency control. Samples in which RNase P is not amplified indicate that sample degradation has occurred, PCR inhibitors are present, RNA extraction has failed or swabbing technique was insufficient. This important internal control reveals that 2.4% of nasopharyngeal swabs (15/618 samples) are inadequate for SARS-CoV-2 testing which, if not identified, could result in false negative results. We show that our assay is linear across at least 7 logs and is highly reproducible, enabling the conversion of Cq values to viral copy numbers using a standard curve. Furthermore, the SARS-CoV-2 copy number was independent of the RNase P copy number indicating that the per-swab viral copy number is not dependent on sampling- further allowing comparisons between samples. The ability to quantify SARS-CoV-2 viral copy number will provide an important opportunity for viral burden-guided public health and clinical decision making.

Journal article

Spencer Chapman M, May PC, Olavarria E, Nadal Melsio Eet al., 2020, Three distinct hematological malignancies from a single germ cell tumor: a case report., Journal of Medical Case Reports, Vol: 14, ISSN: 1752-1947

BACKGROUND: The association between non seminomatous germ cell tumors (GCTs) and hematological malignancies of rare lineage has been described in the literature. In some of these cases there is evidence that the leukemia derives from a pluripotent primitive clone present in the original germ cell tumor. CASE PRESENTATION: We present a highly unusual case of a 23-year-old man of South Asian origin with a history of Klinefelter's syndrome who initially developed mediastinal non seminomatous GCT. Following treatment with surgery and standard chemotherapy he went on to develop three different hematological malignancies of distinct lineages in sequential fashion over a short time period. Despite treatment with multiple intensive chemotherapy regimens and a matched unrelated donor allogeneic stem cell transplant, he died 41 months after initial diagnosis of his GCT and 10 months after the first diagnosis of hematological malignancy. CONCLUSIONS: This is an extreme case that highlights the pluripotency and aggressiveness of these GCT-derived hematological malignancies, and the need for novel therapeutic approaches.

Journal article

Silvestri G, Trotta R, Stramucci L, Ellis JJ, Harb JG, Neviani P, Wang S, Eisfeld A-K, Walker CJ, Zhang B, Srutova K, Gambacorti-Passerini C, Pineda G, Jamieson CHM, Stagno F, Vigneri P, Nteliopoulos G, May PC, Reid AG, Garzon R, Roy D-C, Moutuou MM, Guimond M, Hokland P, Deininger MW, Fitzgerald G, Harman C, Dazzi F, Milojkovic D, Apperley JF, Marcucci G, Qi J, Polakova KM, Zou Y, Fan X, Baer MR, Calabretta B, Perrotti Det al., 2020, Persistence of Drug-Resistant Leukemic Stem Cells and Impaired NK Cell Immunity in CML Patients Depend on MIR300 Antiproliferative and PP2A-Activating Functions., Blood Cancer Discov, Vol: 1, Pages: 48-67

Persistence of drug-resistant quiescent leukemic stem cells (LSC) and impaired natural killer (NK) cell immune response account for relapse of chronic myelogenous leukemia (CML). Inactivation of protein phosphatase 2A (PP2A) is essential for CML-quiescent LSC survival and NK cell antitumor activity. Here we show that MIR300 has antiproliferative and PP2A-activating functions that are dose dependently differentially induced by CCND2/CDK6 and SET inhibition, respectively. MIR300 is upregulated in CML LSCs and NK cells by bone marrow microenvironment (BMM) signals to induce quiescence and impair immune response, respectively. Conversely, BCR-ABL1 downregulates MIR300 in CML progenitors to prevent growth arrest and PP2A-mediated apoptosis. Quiescent LSCs escape apoptosis by upregulating TUG1 long noncoding RNA that uncouples and limits MIR300 function to cytostasis. Genetic and pharmacologic MIR300 modulation and/or PP2A-activating drug treatment restore NK cell activity, inhibit BMM-induced growth arrest, and selectively trigger LSC apoptosis in vitro and in patient-derived xenografts; hence, the importance of MIR300 and PP2A activity for CML development and therapy.

Journal article

Trasanidis N, Alvarez-Benayas J, Katsarou A, Chaidos A, May PC, Ponnusamy K, Xiao X, Bua M, Atta M, Roberts I, Auner HW, Hatjiharissi E, Papaioannou M, Caputo VS, Sudbery I, Karadimitris Aet al., 2019, Distinct Chromatin Accessibility Changes, Aberrant Transcription Factor Networks Combined with Novel Oncogenic Enhancers Characterise Myeloma-Initiating Genetic Events, 61st Annual Meeting and Exposition of the American-Society-of-Hematology (ASH), Publisher: AMER SOC HEMATOLOGY, ISSN: 0006-4971

Conference paper

Innes A, Wooley P, Szydlo R, Lozano S, Fernando F, Bansal D, Palanicawandar R, Milojkovic D, May P, Nadal-Melsio E, Yebra-Fernandez E, Olavarria E, Apperley J, Pavlu Jet al., 2019, Complete remission with incomplete count recovery (CRi) prior to allogeneic HCT for acute myeloid leukaemia is associated with a high non-relapse mortality., Leukemia, Vol: 34, Pages: 667-670, ISSN: 1476-5551

Journal article

Silvestri G, Stramucci L, Ellis J, Harb J, Neviani P, Zhang B, Srutova K, Gambacorti-Passerini C, Pineda G, Jamieson C, Calabretta B, Stagno F, Vigneri P, Nteliopoulos G, May P, Reid A, Garzon R, Roy D-C, Guimond M, Hokland P, Deininger MW, Fitzgerald G, Harman C, Dazzi F, Milojkovic D, Apperley JF, Marcucci G, Qi J, Polakova KM, Fan X, Baer MR, Trotta R, Perrotti Det al., 2017, The Bone Marrow Niche Uses Mir-300 As a Biological Rheostat to Selectively Control Stem Cell-Driven Malignant Hematopoiesis and Innate Anti-Cancer Immunity, 59th Annual Meeting of the American-Society-of-Hematology (ASH), Publisher: AMER SOC HEMATOLOGY, ISSN: 0006-4971

Conference paper

Boulianne B, Robinson ME, May PC, Castellano L, Blighe K, Thomas J, Reid A, Muschen M, Apperley JF, Stebbing J, Feldhahn Net al., 2017, Lineage-specific genes are prominent DNA damage hotspots during leukemic transformation of B-cell precursors, Cell Reports, Vol: 18, Pages: 1687-1698, ISSN: 2211-1247

In human leukemia, lineage-specific genes represent predominant targets of deletion, with lymphoid-specific genes frequently affected in lymphoid leukemia and myeloid-specific genes in myeloid leukemia. To investigate the basis of lineage-specific alterations, we analyzed global DNA damage in primary B-cell precursors expressing leukemia-inducing oncogenes by ChIP-Seq. We identified >1000 sensitive regions, of which B-lineage-specific genes constitute the most prominent targets. Identified hotspots at B-lineage genes relate to DNA-DSBs, affect genes that harbor genomic lesions in human leukemia, and associate with ectopic deletionin successfully transformed cells. We further show that mostidentified regions overlap with gene bodies of highly expressed genes, and that induction of a myeloidlineage phenotype in transformed B-cell precursors promotes de novoDNA damage atmyeloid loci. Hence, we demonstrate thatlineage-specific transcriptionpredisposeslineage-specificgenes in transformed B-cell precursorsto DNA damage, whichis likely to promote the frequent alteration oflineage-specific genes in human leukemia.

Journal article

May PC, Khorashad JS, Alikian M, Perrotti D, Reid Aet al., 2016, The genetics of chronic myelogenous leukaemia, The Genetic Basis of Haematological Cancers, Editors: Reid, Tosi, Publisher: John Wiley & Sons, ISBN: 9781118528051

Written by a team of international experts, this book provides an authoritative overview and practical guide to the molecular biology and genetic basis of haematologic cancers including leukemia.

Book chapter

Sebire NJ, May PC, Kaur B, Seckl MJ, Fisher RAet al., 2016, Abnormal Villous Morphology Mimicking a Hydatidiform Mole Associated with Paternal Trisomy of Chromosomes 3,7,8 and Unipaternal Disomy of Chromosome 11., Diagnostic Pathology, Vol: 11, ISSN: 1746-1596

BackgroundPregnancies affected by non-molar chromosomal abnormality may sometimes demonstrate abnormal chorionic villous morphology that is similar to partial hydatidiform mole. Determination of the underlying aetiology may be difficult in such cases.Case PresentationThis report describes a case referred to the regional trophoblastic disease unit as a possible hydatidiform mole that demonstrated both villous dysmorphology and abnormal p57KIP2 expression. Molecular genotyping revealed that while most chromosomes in the villous tissue were diploid and biparental, chromosomes 3, 7 and 8 were trisomic with an additional paternally derived chromosome. In contrast chromosome 11 showed uniparental disomy of paternal origin a situation more usually associated with complete hydatidiform moles. This unusual case highlights that exceptions may occur to the general rules of both histological morphology and immunoprofile, and that these can be resolved by detailed molecular genetic investigations.ConclusionThe findings confirm that trisomic pregnancies may demonstrate morphological villous features similar to hydatidiform mole, and that loss of p57KIP2 expression occurs due to an absence of maternally transcribed genes on chromosome 11 and can therefore be independent of androgenetic complete hydatidiform mole.

Journal article

Johnston AC, Naresh K, Barwick T, May P, Karadimitris A, Auner HWet al., 2015, Cutaneous presentation of an aggressive plasmablastic neoplasm indiscriminate between lymphoma and myeloma, ANNALS OF HEMATOLOGY, Vol: 94, Pages: 691-692, ISSN: 0939-5555

Journal article

Neelakantan P, Rezvani K, May P, Gerrard G, Marco B, Paliompeis C, Reid A, Goldman J, Marin D, Milojkovic Det al., 2014, Excellent outcome after repeated changes of tyrosine kinase inhibitor therapy for chronic myeloid leukaemia in complete cytogenetic response due to minor side effects, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 164, Pages: 608-610, ISSN: 0007-1048

Journal article

Neviani P, Harb JG, Oaks JJ, Santhanam R, Walker CJ, Ellis JJ, Ferenchak G, Dorrance AM, Paisie CA, Eiring AM, Ma Y, Mao HC, Zhang B, Wunderlich M, May PC, Sun C, Saddoughi SA, Bielawski J, Blum W, Klisovic RB, Solt JA, Byrd JC, Volinia S, Cortes J, Huettner CS, Koschmieder S, Holyoake TL, Devine S, Caligiuri MA, Croce CM, Garzon R, Ogretmen B, Arlinghaus RB, Chen C-S, Bittman R, Hokland P, Roy D-C, Milojkovic D, Apperley J, Goldman JM, Reid A, Mulloy JC, Bhatia R, Marcucci G, Perrotti Det al., 2013, PP2A-activating drugs selectively eradicate TKI-resistant chronic myeloid leukemic stem cells, JOURNAL OF CLINICAL INVESTIGATION, Vol: 123, Pages: 4144-4157, ISSN: 0021-9738

Journal article

Auner HW, Moody AM, Ward TH, Kraus M, Milan E, May P, Chaidos A, Driessen C, Cenci S, Dazzi F, Rahemtulla A, Apperley JF, Karadimitris A, Dillon Net al., 2013, Combined Inhibition of p97 and the Proteasome Causes Lethal Disruption of the Secretory Apparatus in Multiple Myeloma Cells, PLOS One, Vol: 8, ISSN: 1932-6203

Inhibition of the proteasome is a widely used strategy for treating multiple myeloma that takes advantage of the heavy secretory load that multiple myeloma cells (MMCs) have to deal with. Resistance of MMCs to proteasome inhibition has been linked to incomplete disruption of proteasomal endoplasmic-reticulum (ER)-associated degradation (ERAD) and activation of non-proteasomal protein degradation pathways. The ATPase p97 (VCP/Cdc48) has key roles in mediating both ERAD and non-proteasomal protein degradation and can be targeted pharmacologically by small molecule inhibition. In this study, we compared the effects of p97 inhibition with Eeyarestatin 1 and DBeQ on the secretory apparatus of MMCs with the effects induced by the proteasome inhibitor bortezomib, and the effects caused by combined inhibition of p97 and the proteasome. We found that p97 inhibition elicits cellular responses that are different from those induced by proteasome inhibition, and that the responses differ considerably between MMC lines. Moreover, we found that dual inhibition of both p97 and the proteasome terminally disrupts ER configuration and intracellular protein metabolism in MMCs. Dual inhibition of p97 and the proteasome induced high levels of apoptosis in all of the MMC lines that we analysed, including bortezomib-adapted AMO-1 cells, and was also effective in killing primary MMCs. Only minor toxicity was observed in untransformed and non-secretory cells. Our observations highlight non-redundant roles of p97 and the proteasome in maintaining secretory homeostasis in MMCs and provide a preclinical conceptual framework for dual targeting of p97 and the proteasome as a potential new therapeutic strategy in multiple myeloma.

Journal article

Deplano S, May PC, Pavlu J, 2013, Double minutes with MYC amplification in a patient with chronic myelomonocytic leukaemia transformed into acute myeloid leukaemia, BRITISH JOURNAL OF HAEMATOLOGY, Vol: 162, Pages: 720-720, ISSN: 0007-1048

Journal article

Neelakantan P, Gerrard G, Lucas C, Milojkovic D, May P, Wang L, Paliompeis C, Bua M, Reid A, Rezvani K, O'Brien S, Clark R, Goldman J, Marin Det al., 2013, Combining BCR-ABL1 transcript levels at 3 and 6 months in chronic myeloid leukemia: implications for early intervention strategies, BLOOD, Vol: 121, Pages: 2739-2742, ISSN: 0006-4971

Journal article

Ahmed SO, Deplano S, May PC, Bain BJet al., 2013, Observation of auer rods in crushed cells helps in the diagnosis of acute promyelocytic leukemia, AMERICAN JOURNAL OF HEMATOLOGY, Vol: 88, Pages: 236-236, ISSN: 0361-8609

Journal article

Chaidos A, Barnes CP, Cowan G, May PC, Melo V, Hatjiharissi E, Papaioannou M, Harrington H, Doolittle H, Terpos E, Dimopoulos M, Abdalla S, Yarranton H, Naresh K, Foroni L, Reid A, Rahemtulla A, Stumpf M, Roberts I, Karadimitris Aet al., 2013, Clinical drug resistance linked to interconvertible phenotypic and functional states of tumor-propagating cells in multiple myeloma, BLOOD, Vol: 121, Pages: 318-328, ISSN: 0006-4971

Journal article

Innes A, May PC, Pavlu J, Bain BJet al., 2012, Unexpected pancytopenia following treatment of acute lymphoblastic leukemia, AMERICAN JOURNAL OF HEMATOLOGY, Vol: 87, Pages: 412-412, ISSN: 0361-8609

Journal article

Al-Shieban S, Liu C, Fishlock K, May PC, Pavlu J, Naresh KNet al., 2012, Bone marrow trephine biopsy findings in acute promyelocytic leukemia., Am J Hematol, Vol: 87, Pages: 109-110

Journal article

Abdalla S, May PC, Garimberti E, Naresh KNet al., 2011, Bone marrow trephine biopsy findings in myeloma with small-lymphoid cells and CCND1 translocation, AMERICAN JOURNAL OF HEMATOLOGY, Vol: 86, Pages: 1038-1038, ISSN: 0361-8609

Journal article

Schwarb H, May PC, Apperley J, Naresh KNet al., 2011, Bone marrow trephine biopsy findings in B acute lymphoblastic leukaemia of early precursor (Pro-B) subtype, AMERICAN JOURNAL OF HEMATOLOGY, Vol: 86, Pages: 195-196, ISSN: 0361-8609

Journal article

Daghistani M, Marin D, Khorashad JS, Wang L, May PC, Paliompeis C, Milojkovic D, De Melo VA, Gerrard G, Goldman JM, Apperley JF, Clark RE, Foroni L, Reid AGet al., 2010, EVI-1 oncogene expression predicts survival in chronic-phase CML patients resistant to imatinib treated with second-generation tyrosine kinase inhibitors, BLOOD, Vol: 116, Pages: 6014-6017, ISSN: 0006-4971

Journal article

Naresh KN, May PC, Reid AG, Marks AJ, Macdonald D, Kanfer Eet al., 2010, T cell lymphoblastic leukaemia/lymphoma associated with a microenvironment of thymic asteroid B cells in the bone marrow, HISTOPATHOLOGY, Vol: 57, Pages: 549-554, ISSN: 0309-0167

Journal article

Khorashad J, Hu M, Hopmeier D, Daghistani M, May P, Gerrard G, Marin D, Milojkovic D, Apperley J, Foroni L, Goldman J, Karadimitris A, Reid Aet al., 2010, NAMPT, A MEDIATOR OF NORMAL GRANULOCYTIC DIFFERENTIATION, IS DOWN-REGULATED IN CHRONIC MYELOID LEUKAEMIA VIA CHROMATIN MODIFICATION, Publisher: FERRATA STORTI FOUNDATION, Pages: 49-50, ISSN: 0390-6078

Conference paper

Bazeos A, Marin D, Reid AG, Gerrard G, Milojkovic D, May PC, de Lavallade H, Garland P, Rezvani K, Apperley JF, Goldman JM, Foroni L, Khorashad JSet al., 2010, hOCT1 transcript levels and single nucleotide polymorphisms as predictive factors for response to imatinib in chronic myeloid leukemia, LEUKEMIA, Vol: 24, Pages: 1243-1245, ISSN: 0887-6924

Journal article

May PC, Foot N, Dunn R, Geoghegan H, Neat MJet al., 2010, Detection of cryptic and variant IGH-MYC rearrangements in high-grade non-Hodgkin's lymphoma by fluorescence in situ hybridization: implications for cytogenetic testing, CANCER GENETICS AND CYTOGENETICS, Vol: 198, Pages: 71-75, ISSN: 0165-4608

Journal article

May P, Thomas H, Scott R, Mann Ket al., 2007, Reduction of maternal lymphocytes in bloodstained amniotic fluid by centrifugation and subsequent QF-PCR anuploidy testing, British Human Genetics Conference, Publisher: B M J PUBLISHING GROUP, Pages: S107-S107, ISSN: 0022-2593

Conference paper

This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.

Request URL: http://wlsprd.imperial.ac.uk:80/respub/WEB-INF/jsp/search-html.jsp Request URI: /respub/WEB-INF/jsp/search-html.jsp Query String: respub-action=search.html&id=00614977&limit=30&person=true