Imperial College London
Alternate

DrPrashantSrivastava

Faculty of Medicine, National Heart & Lung Institute

Lecturer in Cardiovascular Bioinformatics and Medical Statis
 
 
 
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Contact

 

prashant.srivastava

 
 
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Location

 

337ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Palmisano:2019:10.1038/s41593-019-0490-4,
author = {Palmisano, I and Danzi, MC and Hutson, TH and Zhou, L and McLachlan, E and Serger, E and Shkura, K and Srivastava, PK and Hervera, A and Neill, NO and Liu, T and Dhrif, H and Wang, Z and Kubat, M and Wuchty, S and Merkenschlager, M and Levi, L and Elliott, E and Bixby, JL and Lemmon, VP and Di, Giovanni S},
doi = {10.1038/s41593-019-0490-4},
journal = {Nature Neuroscience},
pages = {1913--1924},
title = {Epigenomic signatures underpin the axonal regenerative ability of dorsal root ganglia sensory neurons},
url = {http://dx.doi.org/10.1038/s41593-019-0490-4},
volume = {22},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Axonal injury results in regenerative success or failure, depending on whether the axon lies in the peripheral or the CNS, respectively. The present study addresses whether epigenetic signatures in dorsal root ganglia discriminate between regenerative and non-regenerative axonal injury. Chromatin immunoprecipitation for the histone 3 (H3) post-translational modifications H3K9ac, H3K27ac and H3K27me3; an assay for transposase-accessible chromatin; and RNA sequencing were performed in dorsal root ganglia after sciatic nerve or dorsal column axotomy. Distinct histone acetylation and chromatin accessibility signatures correlated with gene expression after peripheral, but not central, axonal injury. DNA-footprinting analyses revealed new transcriptional regulators associated with regenerative ability. Machine-learning algorithms inferred the direction of most of the gene expression changes. Neuronal conditional deletion of the chromatin remodeler CCCTC-binding factor impaired nerve regeneration, implicating chromatin organization in the regenerative competence. Altogether, the present study offers the first epigenomic map providing insight into the transcriptional response to injury and the differential regenerative ability of sensory neurons.
AU  - Palmisano,I
AU  - Danzi,MC
AU  - Hutson,TH
AU  - Zhou,L
AU  - McLachlan,E
AU  - Serger,E
AU  - Shkura,K
AU  - Srivastava,PK
AU  - Hervera,A
AU  - Neill,NO
AU  - Liu,T
AU  - Dhrif,H
AU  - Wang,Z
AU  - Kubat,M
AU  - Wuchty,S
AU  - Merkenschlager,M
AU  - Levi,L
AU  - Elliott,E
AU  - Bixby,JL
AU  - Lemmon,VP
AU  - Di,Giovanni S
DO  - 10.1038/s41593-019-0490-4
EP  - 1924
PY  - 2019///
SN  - 1097-6256
SP  - 1913
TI  - Epigenomic signatures underpin the axonal regenerative ability of dorsal root ganglia sensory neurons
T2  - Nature Neuroscience
UR  - http://dx.doi.org/10.1038/s41593-019-0490-4
UR  - https://www.nature.com/articles/s41593-019-0490-4
UR  - http://hdl.handle.net/10044/1/73929
VL  - 22
ER  -
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