Imperial College London
Alternate

DrPrashantSrivastava

Faculty of Medicine, National Heart & Lung Institute

Lecturer in Cardiovascular Bioinformatics and Medical Statis
 
 
 
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Contact

 

prashant.srivastava

 
 
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Location

 

337ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Feuerborn:2015:10.1038/onc.2014.54,
author = {Feuerborn, A and Mathow, D and Srivastava, PK and Gretz, N and Gröne, H-J},
doi = {10.1038/onc.2014.54},
journal = {Oncogene},
pages = {1185--1195},
title = {Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity.},
url = {http://dx.doi.org/10.1038/onc.2014.54},
volume = {34},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Transforming growth factor-β1 (TGF-β1) is a multifunctional cytokine and critically involved in the progression of a variety of cancers. TGF-β1 signaling can impair tumor development by its anti-proliferative and pro-apoptotic features. In contrast, it may actively promote tumor progression and cancer cell dissemination by inducing a gradual switch from epithelial towards mesenchymal-like cell features (EMT-like), including decreased intercellular adhesion. Here, we show that expression of the transcription factor Basonuclin-1 (Bnc1) modulates TGF-β1-induced epithelial dedifferentiation of mammary epithelial cells. RNAi-mediated repression of Bnc1 resulted in enhanced intercellular adhesion and strongly impaired TGF-β1-dependent sheet disintegration and cell scattering. In contrast, forced expression of Bnc1 modifies plasma membrane/cytoskeletal dynamics and seemingly interferes with the initiation of sustainable cell-cell contacts. Follow-up analyses revealed that Bnc1 affects the expression of numerous TGF-β1-responsive genes including distinct EMT-related transcription factors, some of which modulate the expression of Bnc1 themselves. These results suggest that Bnc1 is part of a transcription factor network related to epithelial plasticity with reciprocal feedback-loop connections on which Smad-factors integrate TGF-β1 signaling. Our study demonstrates that Bnc1 regulates epithelial plasticity of mammary epithelial cells and influences outcome of TGF-β1 signaling.
AU  - Feuerborn,A
AU  - Mathow,D
AU  - Srivastava,PK
AU  - Gretz,N
AU  - Gröne,H-J
DO  - 10.1038/onc.2014.54
EP  - 1195
PY  - 2015///
SP  - 1185
TI  - Basonuclin-1 modulates epithelial plasticity and TGF-β1-induced loss of epithelial cell integrity.
T2  - Oncogene
UR  - http://dx.doi.org/10.1038/onc.2014.54
UR  - https://www.ncbi.nlm.nih.gov/pubmed/24662832
VL  - 34
ER  -
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