Publications

BibTex format

@article{Loo:2021:10.1038/s41370-020-0252-0,
author = {Loo, RL and Lu, Q and Carter, E and Liu, S and Clark, S and Wang, Y and Jill, B and Huiru, T and Chan, Q},
doi = {10.1038/s41370-020-0252-0},
journal = {Journal of Exposure Science and Environmental Epidemiology},
pages = {328--344},
title = {A feasibility study of metabolic phenotyping of dried blood spot specimens in rural Chinese women exposed to household air pollution},
url = {http://dx.doi.org/10.1038/s41370-020-0252-0},
volume = {31},
year = {2021}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Background:  Exposure-response studies and policy evaluations of household air pollution (HAP) are limited by current methods of exposure assessment which are expensive and burdensome to participants.Methods: We collected 152 dried blood spot (DBS) specimens during the heating and non-heating seasons from 53 women who regularly used biomass-burning stoves for cooking and heating. Participants were enrolled in a longitudinal study in China. Untargeted metabolic phenotyping of DBS were generated using ultra-high performance liquid chromatography coupled with mass spectrometry to exemplify measurement precision and assessment for feasibility to detect exposure to HAP, evaluated by season (high pollution versus low pollution) and measured personal exposure to fine particulate matter <2.5µm diameters (PM) and black carbon (BC) in the 48-h prior to collecting the DBS specimen.Results: Metabolites e.g., amino acids, acyl-carnitines, lyso-phosphorylcholines, sphinganine, and choline were detected in the DBS specimens. Our approach is capable of detecting the differences in personal exposure to HAP whilst showing high analytical reproducibility, coefficient of variance (CV) <15%, meeting the U.S. Food and Drug Administration criteria.Conclusions: Our results provide a proof of principle that high resolution metabolic phenotypic data can be generated using a simple DBS extraction method thus remote, low-resource settings where the collection of serum and plasma is logistically challengingor infeasible. The analytical run time (19 mins/specimen) is similar to most global phenotyping methods and therefore suitable for large-scale application.
AU  - Loo,RL
AU  - Lu,Q
AU  - Carter,E
AU  - Liu,S
AU  - Clark,S
AU  - Wang,Y
AU  - Jill,B
AU  - Huiru,T
AU  - Chan,Q
DO  - 10.1038/s41370-020-0252-0
EP  - 344
PY  - 2021///
SN  - 1559-0631
SP  - 328
TI  - A feasibility study of metabolic phenotyping of dried blood spot specimens in rural Chinese women exposed to household air pollution
T2  - Journal of Exposure Science and Environmental Epidemiology
UR  - http://dx.doi.org/10.1038/s41370-020-0252-0
UR  - http://hdl.handle.net/10044/1/80876
VL  - 31
ER  -

Download

DrQueenieChan

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)