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@article{Harada:2018:10.1371/journal.pone.0191230,
author = {Harada, S and Hirayama, A and Chan, Q and Kurihara, A and Fukai, K and Iida, M and Kato, S and Sugiyama, D and Kuwabara, K and Takeuchi, A and Akiyama, M and Okamura, T and Ebbels, TMD and Elliott, P and Tomita, M and Sato, A and Suzuki, C and Sugimoto, M and Soga, T and Takebayashi, T},
doi = {10.1371/journal.pone.0191230},
journal = {PLoS ONE},
title = {Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry.},
url = {http://dx.doi.org/10.1371/journal.pone.0191230},
volume = {13},
year = {2018}
}

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TY  - JOUR
AB  - BACKGROUND: Cohort studies with metabolomics data are becoming more widespread, however, large-scale studies involving 10,000s of participants are still limited, especially in Asian populations. Therefore, we started the Tsuruoka Metabolomics Cohort Study enrolling 11,002 community-dwelling adults in Japan, and using capillary electrophoresis-mass spectrometry (CE-MS) and liquid chromatography-mass spectrometry. The CE-MS method is highly amenable to absolute quantification of polar metabolites, however, its reliability for large-scale measurement is unclear. The aim of this study is to examine reproducibility and validity of large-scale CE-MS measurements. In addition, the study presents absolute concentrations of polar metabolites in human plasma, which can be used in future as reference ranges in a Japanese population. METHODS: Metabolomic profiling of 8,413 fasting plasma samples were completed using CE-MS, and 94 polar metabolites were structurally identified and quantified. Quality control (QC) samples were injected every ten samples and assessed throughout the analysis. Inter- and intra-batch coefficients of variation of QC and participant samples, and technical intraclass correlation coefficients were estimated. Passing-Bablok regression of plasma concentrations by CE-MS on serum concentrations by standard clinical chemistry assays was conducted for creatinine and uric acid. RESULTS AND CONCLUSIONS: In QC samples, coefficient of variation was less than 20% for 64 metabolites, and less than 30% for 80 metabolites out of the 94 metabolites. Inter-batch coefficient of variation was less than 20% for 81 metabolites. Estimated technical intraclass correlation coefficient was above 0.75 for 67 metabolites. The slope of Passing-Bablok regression was estimated as 0.97 (95% confidence interval: 0.95, 0.98) for creatinine and 0.95 (0.92, 0.96) for uric acid. Compared to published data from other large cohort measurement platforms, reproducibility of metabolites common
AU  - Harada,S
AU  - Hirayama,A
AU  - Chan,Q
AU  - Kurihara,A
AU  - Fukai,K
AU  - Iida,M
AU  - Kato,S
AU  - Sugiyama,D
AU  - Kuwabara,K
AU  - Takeuchi,A
AU  - Akiyama,M
AU  - Okamura,T
AU  - Ebbels,TMD
AU  - Elliott,P
AU  - Tomita,M
AU  - Sato,A
AU  - Suzuki,C
AU  - Sugimoto,M
AU  - Soga,T
AU  - Takebayashi,T
DO  - 10.1371/journal.pone.0191230
PY  - 2018///
SN  - 1932-6203
TI  - Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry.
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0191230
UR  - http://hdl.handle.net/10044/1/56564
VL  - 13
ER  -

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