Imperial College London
Alternate

Dr Richard H. Barton

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 3014r.barton Website

 
 
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Location

 

SAFB 660Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wijeyesekera:2012:10.1021/pr2010154,
author = {Wijeyesekera, A and Selman, C and Barton, RH and Holmes, E and Nicholson, JK and Withers, DJ},
doi = {10.1021/pr2010154},
journal = {Journal of Proteome Research},
pages = {2224--2235},
title = {Metabotyping of Long-Lived Mice using H-1 NMR Spectroscopy},
url = {http://dx.doi.org/10.1021/pr2010154},
volume = {11},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Significant advances in understanding aging have been achieved through studying model organisms with extended healthy lifespans. Employing 1H NMR spectroscopy, we characterized the plasma metabolic phenotype (metabotype) of three long-lived murine models: 30% dietary restricted (DR), insulin receptor substrate 1 null (Irs1–/–), and Ames dwarf (Prop1df/df). A panel of metabolic differences were generated for each model relative to their controls, and subsequently, the three long-lived models were compared to one another. Concentrations of mobile very low density lipoproteins, trimethylamine, and choline were significantly decreased in the plasma of all three models. Metabolites including glucose, choline, glycerophosphocholine, and various lipids were significantly reduced, while acetoacetate, d-3-hydroxybutyrate and trimethylamine-N-oxide levels were increased in DR compared to ad libitum fed controls. Plasma lipids and glycerophosphocholine were also decreased in Irs1–/– mice compared to controls, as were methionine and citrate. In contrast, high density lipoproteins and glycerophosphocholine were increased in Ames dwarf mice, as were methionine and citrate. Pairwise comparisons indicated that differences existed between the metabotypes of the different long-lived mice models. Irs1–/– mice, for example, had elevated glucose, acetate, acetone, and creatine but lower methionine relative to DR mice and Ames dwarfs. Our study identified several potential candidate biomarkers directionally altered across all three models that may be predictive of longevity but also identified differences in the metabolic signatures. This comparative approach suggests that the metabolic networks underlying lifespan extension may not be exactly the same for each model of longevity and is consistent with multifactorial control of the aging process.
AU  - Wijeyesekera,A
AU  - Selman,C
AU  - Barton,RH
AU  - Holmes,E
AU  - Nicholson,JK
AU  - Withers,DJ
DO  - 10.1021/pr2010154
EP  - 2235
PY  - 2012///
SN  - 1535-3893
SP  - 2224
TI  - Metabotyping of Long-Lived Mice using H-1 NMR Spectroscopy
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/pr2010154
UR  - http://hdl.handle.net/10044/1/52171
VL  - 11
ER  -
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