Imperial College London
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DrRashedaChowdhury

Faculty of Medicine, National Heart & Lung Institute

Advanced Research Fellow
 
 
 
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r.chowdhury

 
 
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ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gorelik:2020:10.1016/j.ebiom.2020.102845,
author = {Gorelik, J and Sanchez-Alonso, J and Loucks, A and Schobesberger, S and van, Cromvoirt A and Poulet, C and Chowdhury, R and Trayanova, N},
doi = {10.1016/j.ebiom.2020.102845},
journal = {EBioMedicine},
pages = {1--14},
title = {Nanoscale regulation of L-type calcium channels differentiates between ischemic and dilated cardiomyopathies.},
url = {http://dx.doi.org/10.1016/j.ebiom.2020.102845},
volume = {57},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundSubcellular localization and function of L-type calcium channels (LTCCs) play an important role in regulating contraction of cardiomyocytes. Understanding how this is affected by the disruption of transverse tubules during heart failure could lead to new insights into the disease.MethodsCardiomyocytes were isolated from healthy donor hearts, as well as from patients with cardiomyopathies and with left ventricular assist devices. Scanning ion conductance and confocal microscopy was used to study membrane structures in the cells. Super-resolution scanning patch-clamp was used to examine LTCC function in different microdomains. Computational modeling predicted the impact of these changes to arrhythmogenesis at the whole-heart level.FindingsWe showed that loss of structural organization in failing myocytes leads to re-distribution of functional LTCCs from the T-tubules to the sarcolemma. In ischemic cardiomyopathy, the increased LTCC open probability in the T-tubules depends on the phosphorylation by protein kinase A, whereas in dilated cardiomyopathy, the increased LTCC opening probability in the sarcolemma results from enhanced phosphorylation by calcium-calmodulin kinase II. LVAD implantation corrected LTCCs pathophysiological activity, although it did not improve their distribution. Using computational modeling in a 3D anatomically-realistic human ventricular model, we showed how LTCC location and activity can trigger heart rhythm disorders of different severity.InterpretationOur findings demonstrate that LTCC redistribution and function differentiate between disease aetiologies. The subcellular changes observed in specific microdomains could be the consequence of the action of distinct protein kinases.FundingThis work was supported by NIH grant (ROI-HL 126802 to NT-JG) and British Heart Foundation (grant RG/17/13/33173 to JG, project grant PG/16/17/32069 to RAC). Funders had no role in study design, data collection, data analysis, interpretation, writing
AU  - Gorelik,J
AU  - Sanchez-Alonso,J
AU  - Loucks,A
AU  - Schobesberger,S
AU  - van,Cromvoirt A
AU  - Poulet,C
AU  - Chowdhury,R
AU  - Trayanova,N
DO  - 10.1016/j.ebiom.2020.102845
EP  - 14
PY  - 2020///
SN  - 2352-3964
SP  - 1
TI  - Nanoscale regulation of L-type calcium channels differentiates between ischemic and dilated cardiomyopathies.
T2  - EBioMedicine
UR  - http://dx.doi.org/10.1016/j.ebiom.2020.102845
UR  - https://www.sciencedirect.com/science/article/pii/S2352396420302206?via%3Dihub
UR  - http://hdl.handle.net/10044/1/80858
VL  - 57
ER  -
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