Publications
425 results found
Feola AJ, Nelson ES, Myers J, et al., 2018, The Impact of Choroidal Swelling on Optic Nerve Head Deformation, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 59, Pages: 4172-4181, ISSN: 0146-0404
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- Citations: 29
Feola A, Sherwood J, Overby DR, et al., 2018, Impact of Estrogen Deficiency on Outflow Facility and Ocular Biomechanics in Rats, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Ethier CR, Wang K, Li G, et al., 2018, Trabecular meshwork stiffness and outflow resistance are related, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Johnstone MA, Ethier CR, Acott TS, et al., 2018, Collector Channel Dynamics: OCT Capture of Real-time Pressure-dependent Changes in Lumen Area in Ex Vivo Normal and Glaucomatous Eyes, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
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- Citations: 1
Hannon B, Fu J, Read AT, et al., 2018, <i>In vivo</i> assessment of visual acuity following genipin-induced scleral crosslinking, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Keller KE, Bhattacharya SK, Borras T, et al., 2018, Consensus recommendations for trabecular meshwork cell isolation, characterization and culture, EXPERIMENTAL EYE RESEARCH, Vol: 171, Pages: 164-173, ISSN: 0014-4835
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- Citations: 167
Wang K, Li G, Read AT, et al., 2018, The relationship between outflow resistance and trabecular meshwork stiffness in mice, SCIENTIFIC REPORTS, Vol: 8, ISSN: 2045-2322
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- Citations: 45
Snider EJ, Vannatta RT, Schildmeyer L, et al., 2018, Characterizing differences between MSCs and TM cells: Toward autologous stem cell therapies for the glaucomatous trabecular meshwork, JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol: 12, Pages: 695-704, ISSN: 1932-6254
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- Citations: 12
Mulvihill JJE, Raykin J, Snider EJ, et al., 2018, Development of a Platform for Studying 3D Astrocyte Mechanobiology: Compression of Astrocytes in Collagen Gels, ANNALS OF BIOMEDICAL ENGINEERING, Vol: 46, Pages: 365-374, ISSN: 0090-6964
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- Citations: 10
Yun H, Wang Y, Zhou Y, et al., 2018, Human stem cells home to and repair laser-damaged trabecular meshwork in a mouse model, COMMUNICATIONS BIOLOGY, Vol: 1
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- Citations: 22
Campbell IC, Sherwood JM, Overby DR, et al., 2017, Quantification of Scleral Biomechanics and Collagen Fiber Alignment., Glaucoma. Methods in Molecular Biology., Editors: Jakobs, Publisher: Humana Press, Pages: 135-159
The stiffness of the sclera is important in several ocular disorders, and there is hence a need to quantify the biomechanical properties of this tissue. Here, we present two methods for measuring the stiffness of scleral ocular tissues: ocular compliance testing and digital image correlation strain mapping. In tandem with these approaches, we provide two methods to spatially quantify the anisotropic alignment of collagen fibers making up the sclera, using second harmonic generation microscopy and small-angle light scattering. Together, these approaches allow specimen-specific measurement of tissue stiffness and collagen alignment, which are key factors in determining how the eye responds to mechanical loads.
Perea D, Guiu J, Hudry B, et al., 2017, Ret receptor tyrosine kinase sustains proliferation and tissue maturation in intestinal epithelia., EMBO Journal, Vol: 36, Pages: 3029-3045, ISSN: 0261-4189
Expression of the Ret receptor tyrosine kinase is a defining feature of enteric neurons. Its importance is underscored by the effects of its mutation in Hirschsprung disease, leading to absence of gut innervation and severe gastrointestinal symptoms. We report a new and physiologically significant site of Ret expression in the intestine: the intestinal epithelium. Experiments in Drosophila indicate that Ret is expressed both by enteric neurons and adult intestinal epithelial progenitors, which require Ret to sustain their proliferation. Mechanistically, Ret is engaged in a positive feedback loop with Wnt/Wingless signalling, modulated by Src and Fak kinases. We find that Ret is also expressed by the developing intestinal epithelium of mice, where its expression is maintained into the adult stage in a subset of enteroendocrine/enterochromaffin cells. Mouse organoid experiments point to an intrinsic role for Ret in promoting epithelial maturation and regulating Wnt signalling. Our findings reveal evolutionary conservation of the positive Ret/Wnt signalling feedback in both developmental and homoeostatic contexts. They also suggest an epithelial contribution to Ret loss-of-function disorders such as Hirschsprung disease.
Wang K, Johnstone MA, Xin C, et al., 2017, Estimating Human Trabecular Meshwork Stiffness by Numerical Modeling and Advanced OCT Imaging, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 58, Pages: 4809-4817, ISSN: 0146-0404
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- Citations: 49
Nelson ES, Mulugeta L, Feola A, et al., 2017, The impact of ocular hemodynamics and intracranial pressure on intraocular pressure during acute gravitational changes, JOURNAL OF APPLIED PHYSIOLOGY, Vol: 123, Pages: 352-363, ISSN: 8750-7587
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- Citations: 19
Wang K, Read AT, Li G, et al., 2017, Effects of dexamethasone on trabecular meshwork stiffness in mice, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Read AT, Overby DR, Ethier CR, 2017, A Method to Expose the Inner Wall of Schlemm's Canal in Mice, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) - Imaging in the Eye, Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Madekurozwa M, Feola A, Ethier CR, et al., 2017, Comparison of Pressure-Dependent Facility in Rodent Eyes, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO) - Imaging in the Eye, Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Feola A, Samuels BC, Nelson E, et al., 2017, The Impact of Choroidal Swelling on Optic Nerve Head Deformation, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Hannon B, Campbell I, Read AT, et al., 2017, Determining the Efficacy of Crosslinking Stiffening Agents in Rat Sclera, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Ethier CR, Snider E, Kubelick K, et al., 2017, Visualizing stem cell delivery to trabecular meshwork using ultrasound/photoacoustic imaging with nanoparticles, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Fu J, Allen RS, Campbell I, et al., 2017, Menopause alters Ocular Biomechanics and Increases Visual Impairment in a Rat Model of Glaucoma, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Overby DR, Braakman S, Spenlehauer A, et al., 2017, Stretch-Dependent Pore Formation in Glaucomatous Schlemm's Canal Cells, Annual Meeting of the Association-for-Research-in-Vision-and-Ophthalmology (ARVO), Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404
Chang JYH, Chow LW, Dismuke WM, et al., 2017, Peptide-functionalized fluorescent particles for in situ detection of nitric oxide via peroxynitrite-mediated nitration, Advanced Healthcare Materials, Vol: 6, ISSN: 2192-2640
Nitric oxide (NO) is a free radical signaling molecule that plays a crucial role in modulating physiological homeostasis across multiple biological systems. NO dysregulation is linked to the pathogenesis of multiple diseases; therefore, its quantification is important for understanding pathophysiological processes. The detection of NO is challenging, typically limited by its reactive nature and short half-life. Additionally, the presence of interfering analytes and accessibility to biological fluids in the native tissues make the measurement technically challenging and often unreliable. Here, a bio-inspired peptide-based NO sensor is developed, which detects NO-derived oxidants, predominately peroxynitrite-mediated nitration of tyrosine residues. It is demonstrated that these peptide-based NO sensors can detect peroxynitrite-mediated nitration in response to physiological shear stress by endothelial cells in vitro. Using the peptide-conjugated fluorescent particle immunoassay, peroxynitrite-mediated nitration activity with a detection limit of ≈100 × 10−9m is detected. This study envisions that the NO detection platform can be applied to a multitude of applications including monitoring of NO activity in healthy and diseased tissues, localized detection of NO production of specific cells, and cell-based/therapeutic screening of peroxynitrite levels to monitor pronitroxidative stress in biological samples.
Wang K, Read AT, Sulchek T, et al., 2017, Trabecular meshwork stiffness in glaucoma, EXPERIMENTAL EYE RESEARCH, Vol: 158, Pages: 3-12, ISSN: 0014-4835
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- Citations: 79
Campbell IC, Hannon BG, Read AT, et al., 2017, Quantification of the efficacy of collagen cross-linking agents to induce stiffening of rat sclera (vol 14, 20170014, 2017), Journal of the Royal Society Interface, Vol: 14, ISSN: 1742-5662
The concept of scleral stiffening therapies has emerged as a novel theoretical approach for treating the ocular disorders glaucoma and myopia. Deformation of specific regions of the posterior eye is innately involved in the pathophysiology of these diseases, and thus targeted scleral stiffening could resist these changes and slow or prevent progression of these diseases. Here, we present the first systematic screen and direct comparison of the stiffening effect of small molecule collagen cross-linking agents in the posterior globe, namely using glyceraldehyde, genipin and methylglyoxal (also called pyruvaldehyde). To establish a dose–response relationship, we used inflation testing to simulate the effects of increasing intraocular pressure in freshly harvested rat eyes stiffened with multiple concentrations of each agent. We used digital image correlation to compute the mechanical strain in the tissue as a metric of stiffness, using a novel treatment paradigm for screening relative stiffening by incubating half of each eye in cross-linker and using the opposite half as an internal control. We identified the doses necessary to increase stiffness by approximately 100%, namely 30 mM for glyceraldehyde, 1 mM for genipin and 7 mM for methylglyoxal, and we also identified the range of stiffening it was possible to achieve with such agents. Such findings will inform development of in vivo studies of scleral stiffening to treat glaucoma and myopia.
Feola AJ, Coudrillier B, Mulvihill J, et al., 2017, Deformation of the Lamina Cribrosa and Optic Nerve Due to Changes in Cerebrospinal Fluid Pressure, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 58, Pages: 2070-2078, ISSN: 0146-0404
Tamm ER, Ethier CR, 2017, Biological aspects of axonal damage in glaucoma: A brief review, EXPERIMENTAL EYE RESEARCH, Vol: 157, Pages: 5-12, ISSN: 0014-4835
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- Citations: 48
Stowell C, Burgoyne CF, Tamm ER, et al., 2017, Biomechanical aspects of axonal damage in glaucoma: A brief review, EXPERIMENTAL EYE RESEARCH, Vol: 157, Pages: 13-19, ISSN: 0014-4835
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- Citations: 53
Raykin J, Snider E, Bheri S, et al., 2017, A modified gelatin zymography technique incorporating total protein normalization, ANALYTICAL BIOCHEMISTRY, Vol: 521, Pages: 8-10, ISSN: 0003-2697
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- Citations: 8
Raykin J, Forte TE, Wang R, et al., 2017, Characterization of the mechanical behavior of the optic nerve sheath and its role in spaceflight-induced ophthalmic changes, BIOMECHANICS AND MODELING IN MECHANOBIOLOGY, Vol: 16, Pages: 33-43, ISSN: 1617-7959
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- Citations: 18
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