Imperial College London
Alternate

Professor C. Ross Ethier

Faculty of Engineering, Department of Bioengineering

Visiting Professor
 
 
 
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Contact

 

r.ethier

 
 
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Location

 

Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Amin:2015:10.1007/s00441-015-2292-7,
author = {Amin, HD and Ethier, CR},
doi = {10.1007/s00441-015-2292-7},
journal = {Cell and Tissue Research},
pages = {219--224},
title = {Differential effects of tyrosine-rich amelogenin peptide on chondrogenic and osteogenic differentiation of adult chondrocytes},
url = {http://dx.doi.org/10.1007/s00441-015-2292-7},
volume = {364},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Current approaches to treat osteoarthritis (OA) are insufficient. Autologous chondrocyte implantation (ACI) has been used for the past decade to treat patients with OA or focal cartilage defects. However, a number of complications have been reported post-ACI, including athrofibrosis and symptomatic hypertrophy. Thus, a long term ACI strategy should ideally incorporate methods to ‘prime’ autologous chondrocytes to form cartilage-specific matrix and suppress hypertrophic mineralization. The objective of this study was to examine the effects of tyrosine rich amelogenin peptide (TRAP; an isoform of the develop mental protein amelogenin) on human articular cartilage cell (HAC) chondrogenic differentiation and hypertrophic mineralization in vitro. Effects of chemically synthesized TRAP on HAC chondrogenic differentiation were determined by assessing: (i) sGAG production; (ii) Alcian blue staining for proteoglycans; (iii) Collagen type II immunostaining; and (iv) Expression of the chondrogenic genes SOX9, ACAN and COL2A1. Hypertrophic mineralization was assayed by: (i) ALP expression; (ii) Alizarin red staining for Ca+2 -rich bone nodules; (iii) OC immunostaining; and (iv) Expression of the Osteogenic/Hypertrophic genes Ihh and BSP. Chemically synthesized TRAP was found to suppress terminal osteogenic differentiation of HACs cultured in hype rtrophic mineralization-like conditions, an effect mediated via down regulation of the Ihh gene. Moreover, TRAP was found to augment chondrogenic differentiation of HACs via induction of SOX9 gene expression when cells were cultured in pro-chondrogenic media. The results obtained from this proof of concept study motivate further studies on the use of TRAP as part of a preconditioning regimen in autologous chondrocyte implantation procedures for OA patients and patients suffering from focal cartilage defects.
AU  - Amin,HD
AU  - Ethier,CR
DO  - 10.1007/s00441-015-2292-7
EP  - 224
PY  - 2015///
SN  - 1432-0878
SP  - 219
TI  - Differential effects of tyrosine-rich amelogenin peptide on chondrogenic and osteogenic differentiation of adult chondrocytes
T2  - Cell and Tissue Research
UR  - http://dx.doi.org/10.1007/s00441-015-2292-7
UR  - http://hdl.handle.net/10044/1/26684
VL  - 364
ER  -
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