Imperial College London
Alternate

Professor C. Ross Ethier

Faculty of Engineering, Department of Bioengineering

Visiting Professor
 
 
 
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Contact

 

r.ethier

 
 
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Location

 

Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Campbell:2017:10.1098/rsif.2017.0312,
author = {Campbell, IC and Hannon, BG and Read, AT and Sherwood, JM and Schwaner, SA and Ethier, CR},
doi = {10.1098/rsif.2017.0312},
journal = {Journal of the Royal Society Interface},
title = {Quantification of the efficacy of collagen cross-linking agents to induce stiffening of rat sclera (vol 14, 20170014, 2017)},
url = {http://dx.doi.org/10.1098/rsif.2017.0312},
volume = {14},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The concept of scleral stiffening therapies has emerged as a novel theoretical approach for treating the ocular disorders glaucoma and myopia. Deformation of specific regions of the posterior eye is innately involved in the pathophysiology of these diseases, and thus targeted scleral stiffening could resist these changes and slow or prevent progression of these diseases. Here, we present the first systematic screen and direct comparison of the stiffening effect of small molecule collagen cross-linking agents in the posterior globe, namely using glyceraldehyde, genipin and methylglyoxal (also called pyruvaldehyde). To establish a dose–response relationship, we used inflation testing to simulate the effects of increasing intraocular pressure in freshly harvested rat eyes stiffened with multiple concentrations of each agent. We used digital image correlation to compute the mechanical strain in the tissue as a metric of stiffness, using a novel treatment paradigm for screening relative stiffening by incubating half of each eye in cross-linker and using the opposite half as an internal control. We identified the doses necessary to increase stiffness by approximately 100%, namely 30 mM for glyceraldehyde, 1 mM for genipin and 7 mM for methylglyoxal, and we also identified the range of stiffening it was possible to achieve with such agents. Such findings will inform development of in vivo studies of scleral stiffening to treat glaucoma and myopia.
AU  - Campbell,IC
AU  - Hannon,BG
AU  - Read,AT
AU  - Sherwood,JM
AU  - Schwaner,SA
AU  - Ethier,CR
DO  - 10.1098/rsif.2017.0312
PY  - 2017///
SN  - 1742-5662
TI  - Quantification of the efficacy of collagen cross-linking agents to induce stiffening of rat sclera (vol 14, 20170014, 2017)
T2  - Journal of the Royal Society Interface
UR  - http://dx.doi.org/10.1098/rsif.2017.0312
UR  - http://hdl.handle.net/10044/1/52209
VL  - 14
ER  -
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