Imperial College London
Alternate

ProfessorRichardFestenstein

Faculty of Medicine, Department of Brain Sciences

Clinical Professor of Molecular Medicine
 
 
 
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Contact

 

+44 (0)20 3313 8310r.festenstein

 
 
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Assistant

 

Ms Hyacinth Henry +44 (0)20 3313 3172

 
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Location

 

London Institute of Medical Sciences, Mansfield BuildingNeptune BuildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Guillemette:2011:10.1371/journal.pgen.1001354,
author = {Guillemette, B and Drogaris, P and Lin, H-HS and Armstrong, H and Hiragami-Hamada, K and Imhof, A and Bonneil, E and Thibault, P and Verreault, A and Festenstein, RJ},
doi = {10.1371/journal.pgen.1001354},
journal = {PLoS Genetics},
pages = {1--16},
title = {H3 lysine 4 8 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation},
url = {http://dx.doi.org/10.1371/journal.pgen.1001354},
volume = {7},
year = {2011}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Methylation of histone H3 lysine 4 (H3K4me) is an evolutionarily conserved modification whose role in the regulation of gene expression has been extensively studied. In contrast, the function of H3K4 acetylation (H3K4ac) has received little attention because of a lack of tools to separate its function from that of H3K4me. Here we show that, in addition to being methylated, H3K4 is also acetylated in budding yeast. Genetic studies reveal that the histone acetyltransferases (HATs) Gcn5 and Rtt109 contribute to H3K4 acetylation in vivo. Whilst removal of H3K4ac from euchromatin mainly requires the histone deacetylase (HDAC) Hst1, Sir2 is needed for H3K4 deacetylation in heterochomatin. Using genome-wide chromatin immunoprecipitation (ChIP), we show that H3K4ac is enriched at promoters of actively transcribed genes and located just upstream of H3K4 tri-methylation (H3K4me3), a pattern that has been conserved in human cells. We find that the Set1-containing complex (COMPASS), which promotes H3K4me2 and -me3, also serves to limit the abundance of H3K4ac at gene promoters. In addition, we identify a group of genes that have high levels of H3K4ac in their promoters and are inadequately expressed in H3-K4R, but not in set1Δ mutant strains, suggesting that H3K4ac plays a positive role in transcription. Our results reveal a novel regulatory feature of promoter-proximal chromatin, involving mutually exclusive histone modifications of the same histone residue (H3K4ac and H3K4me).
AU  - Guillemette,B
AU  - Drogaris,P
AU  - Lin,H-HS
AU  - Armstrong,H
AU  - Hiragami-Hamada,K
AU  - Imhof,A
AU  - Bonneil,E
AU  - Thibault,P
AU  - Verreault,A
AU  - Festenstein,RJ
DO  - 10.1371/journal.pgen.1001354
EP  - 16
PY  - 2011///
SN  - 1553-7390
SP  - 1
TI  - H3 lysine 4 8 is acetylated at active gene promoters and is regulated by H3 lysine 4 methylation
T2  - PLoS Genetics
UR  - http://dx.doi.org/10.1371/journal.pgen.1001354
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000288996600035&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1001354
UR  - http://hdl.handle.net/10044/1/77203
VL  - 7
ER  -
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