Imperial College London
Alternate

Dr Roya E Haghighat-Khah

Faculty of Natural Sciences, Department of Life Sciences

Honorary Research Associate
 
 
 
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Contact

 

r.haghighat-khah

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Haghighat-Khah:2015:10.1371/journal.pone.0121097,
author = {Haghighat-Khah, RE and Scaife, S and Martins, S and St, John O and Matzen, KJ and Morrison, N and Alphey, L},
doi = {10.1371/journal.pone.0121097},
journal = {PLoS One},
pages = {1--16},
title = {Site-specific cassette exchange systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth},
url = {http://dx.doi.org/10.1371/journal.pone.0121097},
volume = {10},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Genetically engineered insects are being evaluated as potential tools to decrease the economic and public health burden of mosquitoes and agricultural pest insects. Here we describe a new tool for the reliable and targeted genome manipulation of pest insects for research and field release using recombinase mediated cassette exchange (RMCE) mechanisms. We successfully demonstrated the established ΦC31-RMCE method in the yellow fever mosquito, Aedes aegypti, which is the first report of RMCE in mosquitoes. A new variant of this RMCE system, called iRMCE, combines the ΦC31-att integration system and Cre or FLP-mediated excision to remove extraneous sequences introduced as part of the site-specific integration process. Complete iRMCE was achieved in two important insect pests, Aedes aegypti and the diamondback moth, Plutella xylostella, demonstrating the transferability of the system across a wide phylogenetic range of insect pests.
AU  - Haghighat-Khah,RE
AU  - Scaife,S
AU  - Martins,S
AU  - St,John O
AU  - Matzen,KJ
AU  - Morrison,N
AU  - Alphey,L
DO  - 10.1371/journal.pone.0121097
EP  - 16
PY  - 2015///
SN  - 1932-6203
SP  - 1
TI  - Site-specific cassette exchange systems in the Aedes aegypti Mosquito and the Plutella xylostella Moth
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0121097
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000352135600055&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0121097
UR  - http://hdl.handle.net/10044/1/75452
VL  - 10
ER  -
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