Imperial College London
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DrRichardKelwick

Faculty of Medicine, Department of Infectious Disease

Research Associate
 
 
 
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Contact

 

+44 (0)20 7594 3058r.kelwick Website

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Kelwick:2020:10.1101/2020.06.18.159921,
author = {Kelwick, RJR and Webb, AJ and Wang, Y and Heliot, A and Allan, F and Emery, AM and Templeton, MR and Freemont, PS},
doi = {10.1101/2020.06.18.159921},
title = {AL-PHA beads: bioplastic-based protease biosensors for global health applications},
url = {http://dx.doi.org/10.1101/2020.06.18.159921},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>ABSTRACT</jats:title><jats:p>Proteases are multi-functional proteolytic enzymes that have complex roles in human health and disease. Therefore, the development of protease biosensors can be beneficial to global health applications. To this end, we developed Advanced proteoLytic detector PolyHydroxyAlkanoates (AL-PHA) beads – a library of over 20 low-cost, biodegradable, bioplastic-based protease biosensors. Broadly, these biosensors utilise PhaC-reporter fusion proteins that are bound to microbially manufactured polyhydroxyalkanoate beads. In the presence of a specific protease, superfolder green fluorescent reporter proteins are cleaved from the AL-PHA beads - resulting in a loss of bead fluorescence. The Tobacco Etch Virus (TEV) AL-PHA biosensor detected the proteolytic activity of at least 1.85 pM of AcTEV. AL-PHA beads were also engineered to detect cercarial elastase from<jats:italic>Schistosoma mansoni</jats:italic>-derived cercarial transformation fluid (SmCTF) samples, as well as cancer-associated metalloproteinases in extracellular vesicle and cell-conditioned media samples. We envision that AL-PHA beads could be further developed for use in resource-limited settings.</jats:p>
AU  - Kelwick,RJR
AU  - Webb,AJ
AU  - Wang,Y
AU  - Heliot,A
AU  - Allan,F
AU  - Emery,AM
AU  - Templeton,MR
AU  - Freemont,PS
DO  - 10.1101/2020.06.18.159921
PY  - 2020///
TI  - AL-PHA beads: bioplastic-based protease biosensors for global health applications
UR  - http://dx.doi.org/10.1101/2020.06.18.159921
ER  -
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