Publications
98 results found
Hartley A, Khamis R, Mikhail G, et al., 2016, Female gender and coronary intervention are significant risk factors for cardiac catheterisation-related stroke in a tertiary centre experience over a three year period, Journal of the American College of Cardiology, Vol: 67, Pages: 65-65, ISSN: 1558-3597
Khamis RY, Hughes A, Caga-Anan M, et al., 2016, High serum immunoglobulin G and M levels improve cardiovascular risk discrimination in hypertension: a nested case-control substudy of the Anglo-Scandinavian Cardiac Outcomes Trial, Journal of the American College of Cardiology, Vol: 67, Pages: 1912-1912, ISSN: 1558-3597
Khamis RY, Woollard KJ, Hyde GD, et al., 2016, Near Infrared Fluorescence (NIRF) Molecular Imaging of Oxidized LDL with an Autoantibody in Experimental Atherosclerosis, Scientific Reports, Vol: 6, ISSN: 2045-2322
We aimed to develop a quantitative antibody-based near infrared fluorescence (NIRF) approachfor the imaging of oxidized LDL in atherosclerosis. LO1, a well- characterized monoclonalautoantibody that reacts with malondialdehyde-conjugated LDL, was labeled with a NIRF dye toyield LO1-750. LO1-750 specifically identified necrotic core in ex vivo human coronary lesions.Injection of LO1-750 into high fat (HF) fed atherosclerotic Ldlr-/-mice led to specific focallocalization within the aortic arch and its branches, as detected by fluorescence moleculartomography (FMT) combined with micro-computed tomography (CT). Ex vivo confocalmicroscopy confirmed LO1-750 subendothelial localization of LO1-750 at sites ofatherosclerosis, in the vicinity of macrophages. When compared with a NIRF reporter of MMPactivity (MMPSense-645-FAST), both probes produced statistically significant increases inNIRF signal in the Ldlr-/- model in relation to duration of HF diet. When withdrawing the HFdiet, the reduction in oxLDL accumulation, as demonstrated with LO1-750, was less markedthan the effect seen on MMP activity. In the rabbit, in vivo injected LO1-750 localization wassuccessfully imaged ex vivo in aortic lesions with a customised intra-arterial NIRF detectioncatheter. A partially humanized chimeric LO1-Fab-Cys localized similarly to the parentantibody in murine atheroma showing promise for future translation.
Gerber RT, ASP S, Sachdev B, et al., 2015, Reproducibilty of the syntax score in a real world setting: implications for the choice of contemporary revascularisation strategy, BCS, Pages: A20-A21
Iqbal MB, Khamis R, Ilsley C, et al., 2015, Time-Trend Analyses of Bleeding and Mortality After Primary Percutaneous Coronary Intervention During Out of Working Hours Versus In-Working Hours An Observational Study of 11 466 Patients, CIRCULATION-CARDIOVASCULAR INTERVENTIONS, Vol: 8, ISSN: 1941-7640
Tombetti E, Khamis R, Gopalan D, et al., 2015, CORONARY CT-ANGIOGRAPHY REVEALS THE EXTENT OF CORONARY ARTERY DISEASE IN TAKAYASU ARTERITIS, Publisher: BMJ PUBLISHING GROUP, Pages: 515-515, ISSN: 0003-4967
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- Citations: 1
Khamis R, Woollard K, Kojima C, et al., 2014, A novel immunoglobulin G autoantibody against a cryptic epitope in low density lipoprotein (LDL) revealed in atherosclerosis
Khamis R, Woollard K, Boyle JJ, et al., 2014, A Novel Immunoglobulin G Autoantibody Against a Cryptic Epitope in Low Density Lipoprotein Induces Macrophage Tumour Necrosis Factor Release, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Iqbal MB, Ilsley CD, Mikhail G, et al., 2014, Comparison Of Outcomes For Primary Percutaneous Coronary Intervention During Out Of Working Hours Versus In Working Hours: An Observational Cohort Study Of 11,461 Patients, 26th Annual Symposium on Transcatheter Cardiovascular Therapeutics (TCT), Publisher: ELSEVIER SCIENCE INC, Pages: B9-B9, ISSN: 0735-1097
Khamis R, Woollard K, Boyle J, et al., 2014, A NOVEL IMMUNOGLOBULIN G AUTOANTIBODY AGAINST LOW DENSITY LIPOPROTEIN (LDL) WITH PATHOGENIC FUNCTIONS, Annual Conference of the British-Cardiovascular-Society, Publisher: BMJ PUBLISHING GROUP, Pages: A121-A121, ISSN: 1355-6037
Khamis RY, Woollard KJ, Hyde GD, et al., 2014, DEVELOPMENT OF WHOLE BODY AND INTRAVASCULAR NEAR-INFRARED OPTICAL MOLECULAR IMAGING OF MARKERS OF PLAQUE VULNERABLITY IN ATHEROSCLEROSIS, Annual Conference of the British-Cardiovascular-Society
Warboys CM, de Luca A, Amini N, et al., 2014, Disturbed flow promotes endothelial senescence via a p53-dependent Ppathway, Arteriosclerosis, Thrombosis and Vascular Biology, Vol: 34, Pages: 985-995, ISSN: 1079-5642
Objective—Although atherosclerosis is associated with systemic risk factors such as age, high cholesterol, and obesity, plaque formation occurs predominately at branches and bends that are exposed to disturbed patterns of blood flow. The molecular mechanisms that link disturbed flow–generated mechanical forces with arterial injury are uncertain. To illuminate them, we investigated the effects of flow on endothelial cell (EC) senescence.Approach and Results—LDLR−/− (low-density lipoprotein receptor−/−) mice were exposed to a high-fat diet for 2 to 12 weeks (or to a normal chow diet as a control) before the assessment of cellular senescence in aortic ECs. En face staining revealed that senescence-associated β-galactosidase activity and p53 expression were elevated in ECs at sites of disturbed flow in response to a high-fat diet. By contrast, ECs exposed to undisturbed flow did not express senescence-associated β-galactosidase or p53. Studies of aortae from healthy pigs (aged 6 months) also revealed enhanced senescence-associated β-galactosidase staining at sites of disturbed flow. These data suggest that senescent ECs accumulate at disturbed flow sites during atherogenesis. We used in vitro flow systems to examine whether a causal relationship exists between flow and EC senescence. Exposure of cultured ECs to flow (using either an orbital shaker or a syringe-pump flow bioreactor) revealed that disturbed flow promoted EC senescence compared with static conditions, whereas undisturbed flow reduced senescence. Gene silencing studies demonstrated that disturbed flow induced EC senescence via a p53-p21 signaling pathway. Disturbed flow–induced senescent ECs exhibited reduced migration compared with nonsenescent ECs in a scratch wound closure assay, and thus may be defective for arterial repair. However, pharmacological activation of sirtuin 1 (using resveratrol or SRT1720) protected ECs from disturbed flow&ndas
Khamis R, Woollard K, Hyde G, et al., 2014, NEAR-INFRARED FLUORESCENCE (NIRF) WHOLE BODY AND INTRA-ARTERIAL MOLECULAR IMAGING OF OXIDIZED LDL IN EXPERIMENTAL ATHEROSCLEROSIS, JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol: 63, Pages: A2150-A2150, ISSN: 0735-1097
Iqbal MB, Ilsley CD, Mikhail G, et al., 2014, Acute Myocardial Infarction (TCT-9 to TCT-62), Journal of the American College of Cardiology, Vol: 64
Khamis R, Batty T, Wong I, et al., 2013, Dissecting the protective IgG anti-malondialdehyde-LDL (MDA-LDL) antibody response in a substudy of the Anglo Scandinavian Cardiac Outcomes Trial (ASCOT), European Heart Journal, Vol: 34, Pages: P704-P704, ISSN: 0195-668X
Khamis R, Woollard K, Chang SH, et al., 2013, The Near Infra-Red (NIRF) molecular imaging of oxidised LDL in atherosclerosis with the native autoantibody LO1, and its molecularly expressed cysteine-tagged Fab construct (LO1-Fab-cys), European Heart Journal, Vol: 34, Pages: P262-P262, ISSN: 0195-668X
Khamis R, Batty T, Wong I, et al., 2013, Dissecting the protective IgG anti-malondialdehyde-LDL (MDA-LDL) antibody response in a substudy of the Anglo Scandinavian Cardiac Outcomes Trial (ASCOT), Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 141-141, ISSN: 0195-668X
Khamis R, Woollard K, Chang SH, et al., 2013, The Near Infra-Red (NIRF) molecular imaging of oxidised LDL in atherosclerosis with the native autoantibody LO1, and its molecularly expressed cysteine-tagged Fab construct (LO1-Fab-cys), Congress of the European-Society-of-Cardiology (ESC), Publisher: OXFORD UNIV PRESS, Pages: 29-29, ISSN: 0195-668X
Jackson W, Khamis R, Haskard D, et al., 2013, 165 SCAVENGER RECEPTORS MEDIATE UPTAKE OF MODIFIED LDL BY CIRCULATING BLOOD MONOCYTE SUBSETS: CONSEQUENCES FOR ATHEROSCLEROSIS., Pages: A95-A96, ISSN: 1355-6037
Khamis R, Woollard K, Granger D, et al., 2013, IMAGING BEYOND THE LUMEN: NEAR INFRA-RED IN VIVO MOLECULAR IDENTIFICATION OF OXIDISED LDL IN ATHEROSCLEROSIS USING MAB LO1, AND THE GENERATION AND DEVELOPMENT OF ITS MOLECULARLY EXPRESSED CYSTEINE-TAGGED CHIMERIC FAB CONSTRUCT (LO1-FAB-CYS), Annual Conference of the British-Cardiovascular-Society (BCS), Publisher: BMJ PUBLISHING GROUP, ISSN: 1355-6037
Khamis RY, Shang-Hung C, Boyle J, et al., 2012, The Development of LO1, a Novel IgG Monoclonal Natural Antibody, for the Near Infra-Red (NIRF) Imaging of Oxidised LDL in Atherosclerosis, AHA Scientific Sessions
Khamis RY, Johns M, Thom S, et al., 2012, The ASCOT Anti-oxidised LDL Antibodies Substudy, IgG Anti-malondialdehyde-ldl Antibodies are Independent Predictors of Protection From Cardiovascular Events in a Substudy of the Anglo-scandinavian Cardiac Outcomes Trial, AHA Scientific Sessions
Khamis R, Chang S-H, Boyle J, et al., 2012, Abstract 15370: The Development of LO1, a Novel IgG Monoclonal Natural Antibody, for the Near Infra-Red (NIRF) Imaging of Oxidised LDL in Atherosclerosis, CIRCULATION, Vol: 126, ISSN: 0009-7322
Khamis R, Johns M, Thom S, et al., 2012, The ASCOT Anti-oxidised LDL Antibodies Substudy, IgG Anti-malondialdehyde-ldl Antibodies are Independent Predictors of Protection From Cardiovascular Events in a Substudy of the Anglo-scandinavian Cardiac Outcomes Trial, CIRCULATION, Vol: 126, ISSN: 0009-7322
Khamis RY, Chang S-H, Boyle JJ, et al., 2012, THE GENERATION AND CHARACTERISATION OF LO1: A UNIQUE IGG MONOCLONAL NATURAL ANTIBODY AGAINST OXIDISED LDL (OXLDL), Joint Spring Meeting of the British-Society-for-Cardiovascular-Research (BSCR) and British-Atherosclerosis-Society (BAS), Publisher: BMJ PUBLISHING GROUP, Pages: A6-A6, ISSN: 1355-6037
khamis R, Chang S-H, Boyle JJ, et al., 2012, Early development of LO1, a novel IgG monoclonal natural antibody, for the imaging of oxidised LDL in atherosclerotic plaque, http://wmis2012.omnibooksonline.com/, World Molecular Imaging Conference, Publisher: OMNI
Chang S-H, Johns M, Boyle JJ, et al., 2012, Model IgG Monoclonal Autoantibody-Anti-Idiotype Pair for Dissecting the Humoral Immune Response to Oxidized Low Density Lipoprotein, HYBRIDOMA, Vol: 31, Pages: 87-98, ISSN: 1554-0014
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- Citations: 10
Khamis RY, 2012, Oxford Desk Reference: Acute Medicine, Oxford Desk Reference: Acute Medicine, Editors: Leach, Moore, Bell, ISBN: 9780199565979
Khamis RY, Johns M, Thom S, et al., 2011, IGG ANTI-MALONEDIALDEHYDE-LDL ANTIBODIES ARE ASSOCIATED WITH LOW RISK OF CARDIOVASCULAR EVENTS IN A SUBSTUDY OF THE ANGLO-SCANDINAVIAN CARDIAC OUTCOMES TRIAL (ASCOT), AND ARE UNRELATED TO LDL, CRP LEVELS AND STATIN TREATMENT, HEART, Vol: 97, Pages: 5-5, ISSN: 1355-6037
Savage HO, Gerber RT, Tang K, et al., 2011, Reproducibility of the syntax score (synergy between percutaneous coronary intervention with taxus and cardiac surgery) in a real world setting: implications for the choice of contemporary revascularisation strategy, Circulation, ISSN: 0009-7322
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