Imperial College London
Alternate

Dr. Ramzi Y Khamis MB ChB PhD FESC FRCP

Faculty of Medicine, National Heart & Lung Institute

Reader in Cardiology
 
 
 
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Contact

 

+44 (0)20 7594 6842r.khamis

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hartley:2021:10.3390/antiox10081298,
author = {Hartley, A and Khamis, R},
doi = {10.3390/antiox10081298},
journal = {Antioxidants},
pages = {1--14},
title = {A novel immunoassay for malondialdehyde-conjugated low-density lipoprotein measures dynamic changes in the blood of patients undergoing coronary artery bypass graft surgery},
url = {http://dx.doi.org/10.3390/antiox10081298},
volume = {10},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondial-dehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r=0.74, p <0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable trans-lational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability.
AU  - Hartley,A
AU  - Khamis,R
DO  - 10.3390/antiox10081298
EP  - 14
PY  - 2021///
SN  - 2076-3921
SP  - 1
TI  - A novel immunoassay for malondialdehyde-conjugated low-density lipoprotein measures dynamic changes in the blood of patients undergoing coronary artery bypass graft surgery
T2  - Antioxidants
UR  - http://dx.doi.org/10.3390/antiox10081298
UR  - https://www.mdpi.com/2076-3921/10/8/1298
UR  - http://hdl.handle.net/10044/1/90967
VL  - 10
ER  -
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