Imperial College London
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DrRobertKypta

Faculty of Medicine, Department of Surgery & Cancer

Senior Lecturer in Cancer Biology
 
 
 
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Contact

 

r.kypta Website

 
 
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Location

 

4007Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mazo:2018:10.3390/cancers10120517,
author = {Mazo, C and Orue-Etxebarria, E and Zabalza, I and Vivanco, MDM and Kypta, RM and Beristain, A},
doi = {10.3390/cancers10120517},
journal = {Cancers},
title = {In silico approach for immunohistochemical evaluation of a cytoplasmic marker in breast cancer},
url = {http://dx.doi.org/10.3390/cancers10120517},
volume = {10},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Breast cancer is the most frequently diagnosed cancer in women and the second most common cancer overall, with nearly 1.7 million new cases worldwide every year. Breast cancer patients need accurate tools for early diagnosis and to improve treatment. Biomarkers are increasingly used to describe and evaluate tumours for prognosis, to facilitate and predict response to therapy and to evaluate residual tumor, post-treatment. Here, we evaluate different methods to separate Diaminobenzidine (DAB) from Hematoxylin and Eosin (H&E) staining for Wnt-1, a potential cytoplasmic breast cancer biomarker. A method comprising clustering and Color deconvolution allowed us to recognize and quantify Wnt-1 levels accurately at pixel levels. Experimental validation was conducted using a set of 12,288 blocks of m × n pixels without overlap, extracted from a Tissue Microarray (TMA) composed of 192 tissue cores. Intraclass Correlations (ICC) among evaluators of the data of 0.634 , 0.791 , 0.551 and 0.63 for each Allred class and an average ICC of 0.752 among evaluators and automatic classification were obtained. Furthermore, this method received an average rating of 4.26 out of 5 in the Wnt-1 segmentation process from the evaluators.
AU  - Mazo,C
AU  - Orue-Etxebarria,E
AU  - Zabalza,I
AU  - Vivanco,MDM
AU  - Kypta,RM
AU  - Beristain,A
DO  - 10.3390/cancers10120517
PY  - 2018///
SN  - 2072-6694
TI  - In silico approach for immunohistochemical evaluation of a cytoplasmic marker in breast cancer
T2  - Cancers
UR  - http://dx.doi.org/10.3390/cancers10120517
UR  - https://www.ncbi.nlm.nih.gov/pubmed/30558303
UR  - http://hdl.handle.net/10044/1/65362
VL  - 10
ER  -
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