Imperial College London
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DrRobertMahen

Faculty of Natural Sciences, Department of Physics

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r.mahen

 
 
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Location

 

Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Mahen:2018:10.1371/journal.pbio.2003998,
author = {Mahen, RWJ},
doi = {10.1371/journal.pbio.2003998},
journal = {PLoS Biology},
title = {Stable centrosomal roots disentangle to allow interphase centriole independence},
url = {http://dx.doi.org/10.1371/journal.pbio.2003998},
volume = {16},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The centrosome is a non-membrane bound cellular compartment consisting of two centrioles surrounded by a proteincoat termed the pericentriolar material (PCM). Centrioles generally remain physically associated together (a phenomenoncalled centrosome cohesion), yet how this occurs in the absence of a bounding lipid membrane is unclear. One modelposits that pericentriolar fibres formed from rootletin protein directly link centrioles, yet little is known about thestructure, biophysical properties or assembly kinetics of such fibres. Here I combine live cell imaging of endogenouslytagged rootletin with cell fusion, and find previously unrecognised plasticity in centrosome cohesion. Rootletin formslarge, diffusionally stable, bifurcating fibres, which amass slowly on mature centrioles over many hours from anaphase.Nascent centrioles (procentrioles) in contrast do not form roots, and must be licensed to do so through polo-like kinase 1(PLK1) activity. Transient separation of roots accompanies centriolar repositioning during the interphase, suggesting thatcentrioles organize as independent units, each containing discrete roots. Indeed, forced induction of duplicate centriolepairs allows independent re-shuffling of individual centrioles between the pairs. Thus collectively, these findings suggestthat progressively nucleated polymers mediate the dynamic association of centrioles as either one or two interphasecentrosomes, with implications for the understanding of how non-membrane bound organelles self-organise.
AU  - Mahen,RWJ
DO  - 10.1371/journal.pbio.2003998
PY  - 2018///
SN  - 1544-9173
TI  - Stable centrosomal roots disentangle to allow interphase centriole independence
T2  - PLoS Biology
UR  - http://dx.doi.org/10.1371/journal.pbio.2003998
UR  - http://hdl.handle.net/10044/1/58430
VL  - 16
ER  -
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