Imperial College London

DrRichardNicholas

Faculty of MedicineDepartment of Brain Sciences

Professor of Practice (Neurology)
 
 
 
//

Contact

 

r.nicholas

 
 
//

Location

 

12L12CLab BlockCharing Cross Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Nicholas:2001:10.1046/j.0953-816x.2001.01470.x,
author = {Nicholas, RS and Wing, MG and Compston, A},
doi = {10.1046/j.0953-816x.2001.01470.x},
journal = {Eur J Neurosci},
pages = {959--967},
title = {Nonactivated microglia promote oligodendrocyte precursor survival and maturation through the transcription factor NF-kappa B.},
url = {http://dx.doi.org/10.1046/j.0953-816x.2001.01470.x},
volume = {13},
year = {2001}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - We demonstrate a role for nonactivated rat microglia in the survival and maturation of oligodendrocyte precursor cells (OPCs). Media conditioned by nonactivated microglia increase the number of surviving galactocerebroside(+) (GalC(+)) oligodendrocytes in vitro at 48 h by inhibiting the apoptosis of OPCs and stimulating their maturation to GalC+ oligodendrocytes. These effects are not observed with medium conditioned by microglia activated with interferon-gamma (IFN-gamma). Conditioned medium from nonactivated microglia is associated with upregulation in OPCs of nuclear factor of kappa binding (NF-kappa B) p65 subunit. The use of antisense to the inhibitor of kappa binding (I kappa B) induces p65 subunit activation in OPCs and, in common with medium conditioned by nonactivated microglia, also inhibits OPC apoptosis and promotes cell maturation. Anti-platelet-derived growth factor (PDGF) antibody abolishes this effect even though PDGF-A chain is expressed at similar levels within both nonactivated and IFN-gamma-activated microglia and both conditioned media have similar levels of PDGF-A chain bioactivity. However, only conditioned medium from nonactivated microglia recruit phosphatidyl-3-inositol (PI-3) kinase to the PDGF-alpha receptor and synergise with endogenous PDGF-A chain to increase NF-kappa B activation. These results suggest that, dependent on their state of activation, microglia produce soluble factors that promote oligodendrocyte development through an effect on the PDGF-alpha receptor-signalling pathway.
AU - Nicholas,RS
AU - Wing,MG
AU - Compston,A
DO - 10.1046/j.0953-816x.2001.01470.x
EP - 967
PY - 2001///
SN - 0953-816X
SP - 959
TI - Nonactivated microglia promote oligodendrocyte precursor survival and maturation through the transcription factor NF-kappa B.
T2 - Eur J Neurosci
UR - http://dx.doi.org/10.1046/j.0953-816x.2001.01470.x
UR - https://www.ncbi.nlm.nih.gov/pubmed/11264668
VL - 13
ER -