Imperial College London

DrRichardNicholas

Faculty of MedicineDepartment of Brain Sciences

Professor of Practice (Neurology)
 
 
 
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Contact

 

r.nicholas

 
 
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Location

 

12L12CLab BlockCharing Cross Campus

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Summary

 

Publications

Citation

BibTex format

@article{de:2016:10.1002/acn3.310,
author = {de, Paula Alves Sousa A and Johnson, KR and Nicholas, R and Darko, S and Price, DA and Douek, DC and Jacobson, S and Muraro, PA},
doi = {10.1002/acn3.310},
journal = {Annals of Clinical and Translational Neurology},
pages = {422--433},
title = {Intrathecal T-cell clonal expansions in patients with multiple sclerosis},
url = {http://dx.doi.org/10.1002/acn3.310},
volume = {3},
year = {2016}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - ObjectiveAnalysis of the T-cell receptor (TCR) repertoire in the cerebrospinal fluid (CSF) of patients with multiple sclerosis (MS) can reveal antigen-specific immune responses potentially implicated in the disease process. We applied a new unbiased deep-sequencing method for TCR repertoire analysis to accurately measure and compare receptor diversity and clonal expansions within the peripheral and CSF-trafficking T-cell populations of patients with MS and control individuals with idiopathic intracranial hypertension (IIH).MethodsPaired blood and CSF TCR β-chain libraries from five MS patients and five IIH controls were sequenced, yielding a total of 80 million reads.ResultsAlthough TCR repertoire diversity was greater in the blood and CSF compartments of MS patients when compared with IIH controls, it is notable that the frequency of clonal expansions was also significantly higher in both compartments of MS patients. Highly expanded T-cell clones were enriched in the CSF compartment of MS patients compared to peripheral blood, very few of them were detected in both compartments.InterpretationCollectively, our data provide a proof of principle that private compartmentalized T-cell expansion exists in the intrathecal space of MS patients.
AU - de,Paula Alves Sousa A
AU - Johnson,KR
AU - Nicholas,R
AU - Darko,S
AU - Price,DA
AU - Douek,DC
AU - Jacobson,S
AU - Muraro,PA
DO - 10.1002/acn3.310
EP - 433
PY - 2016///
SN - 2328-9503
SP - 422
TI - Intrathecal T-cell clonal expansions in patients with multiple sclerosis
T2 - Annals of Clinical and Translational Neurology
UR - http://dx.doi.org/10.1002/acn3.310
UR - http://hdl.handle.net/10044/1/34427
VL - 3
ER -