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@article{Nadai:2019:10.3389/fimmu.2019.00717,
author = {Nadai, Y and Held, K and Joseph, S and Ahmed, MIM and Hoffmann, VS and Peterhoff, D and Missange, M and Bauer, A and Joachim, A and Reimer, U and Zerweck, J and McCormack, S and Cope, A and Tatoud, R and Shattock, RJ and Robb, ML and Sandstroem, EG and Hoelscher, M and Maboko, L and Bakari, M and Kroidl, A and Wagner, R and Weber, J and Pollakis, G and Geldmacher, C},
doi = {10.3389/fimmu.2019.00717},
journal = {Frontiers in Immunology},
pages = {1--14},
title = {Envelope-specific recognition patterns of HIV vaccine-induced IgG antibodies are linked to immunogen structure and sequence},
url = {http://dx.doi.org/10.3389/fimmu.2019.00717},
volume = {10},
year = {2019}
}

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TY  - JOUR
AB  - Background: A better understanding of the parameters influencing vaccine-induced IgG recognition of individual antigenic regions and their variants within the HIV Envelope protein (Env) can help to improve design of preventive HIV vaccines.Methods: Env-specific IgG responses were mapped in samples of the UKHVC003 Standard Group (UK003SG, n = 11 from UK) and TaMoVac01 (TMV01, n = 17 from Tanzania) HIV vaccine trials. Both trials consisted of three immunizations with DNA, followed by two boosts with recombinant Modified Vaccinia Virus Ankara (MVA), either mediating secretion of gp120 (UK003SG) or the presentation of cell membrane bound gp150 envelopes (TMV01) from infected cells, and an additional two boosts with 5 μg of CN54gp140 protein adjuvanted with glucopyranosyl lipid adjuvant (GLA). Env immunogen sequences in UK003SG were solely based on the clade C isolate CN54, whereas in TMV01 these were based on clades A, C, B, and CRF01AE. The peptide microarray included 8 globally representative Env sequences, CN54gp140 and the MVA-encoded Env immunogens from both trials, as well as additional peptide variants for hot spots of immune recognition.Results: After the second MVA boost, UK003SG vaccinees almost exclusively targeted linear, non-glycosylated antigenic regions located in the inter-gp120 interface. In contrast, TMV01 recipients most strongly targeted the V2 region and an immunodominant region in gp41. The V3 region was frequently targeted in both trials, with a higher recognition magnitude for diverse antigenic variants observed in the UK003SG (p < 0.0001). After boosting with CN54gp140/GLA, the overall response magnitude increased with a more comparable recognition pattern of antigenic regions and variants between the two trials. Recognition of most immunodominant regions within gp120 remained significantly stronger in UK003SG, whereas V2-region recognition was not boosted in either group.Conclusions: IgG recognition of linear antigenic Env regions differe
AU  - Nadai,Y
AU  - Held,K
AU  - Joseph,S
AU  - Ahmed,MIM
AU  - Hoffmann,VS
AU  - Peterhoff,D
AU  - Missange,M
AU  - Bauer,A
AU  - Joachim,A
AU  - Reimer,U
AU  - Zerweck,J
AU  - McCormack,S
AU  - Cope,A
AU  - Tatoud,R
AU  - Shattock,RJ
AU  - Robb,ML
AU  - Sandstroem,EG
AU  - Hoelscher,M
AU  - Maboko,L
AU  - Bakari,M
AU  - Kroidl,A
AU  - Wagner,R
AU  - Weber,J
AU  - Pollakis,G
AU  - Geldmacher,C
DO  - 10.3389/fimmu.2019.00717
EP  - 14
PY  - 2019///
SN  - 1664-3224
SP  - 1
TI  - Envelope-specific recognition patterns of HIV vaccine-induced IgG antibodies are linked to immunogen structure and sequence
T2  - Frontiers in Immunology
UR  - http://dx.doi.org/10.3389/fimmu.2019.00717
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000465381000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.frontiersin.org/articles/10.3389/fimmu.2019.00717/full
UR  - http://hdl.handle.net/10044/1/80742
VL  - 10
ER  -

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