Imperial College London
Alternate

Emeritus ProfessorRobertSinden

Faculty of Natural Sciences, Department of Life Sciences

Emeritus Professor of Parasite Cell Biology
 
 
 
//

Contact

 

r.sinden Website

 
 
//

Location

 

2.1Centre for Population BiologySilwood Park

//

Summary

 

Publications

Citation

BibTex format

@article{Blagborough:2016:10.1016/j.vaccine.2016.05.007.,
author = {Blagborough, AM and Musiychuk, K and Bi, H and Jones, RM and Chichester, JA and Streatfield, S and Sala, KA and Zakutansky, SE and Upton, LM and Sinden, RE and Brian, I and Biswas, S and Sattabonkot, J and Yusibov, V},
doi = {10.1016/j.vaccine.2016.05.007.},
journal = {Vaccine},
pages = {3252--3259},
title = {Transmission blocking potency and immunogenicity of a plant-produced Pvs25-based subunit vaccine against Plasmodium vivax},
url = {http://dx.doi.org/10.1016/j.vaccine.2016.05.007.},
volume = {34},
year = {2016}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Malaria transmission blocking (TB) vaccines (TBVs) directed against proteins expressed on the sexual stages of Plasmodium parasites are a potentially effective means to reduce transmission. Antibodies induced by TBVs block parasite development in the mosquito, and thus inhibit transmission to further human hosts. The ookinete surface protein P25 is a primary target for TBV development. Recently, transient expression in plants using hybrid viral vectors has demonstrated potential as a strategy for cost-effective and scalable production of recombinant vaccines. Using a plant virus-based expression system, we produced recombinant P25 protein of Plasmodium vivax (Pvs25) in Nicotiana benthamiana fused to a modified lichenase carrier protein. This candidate vaccine, Pvs25-FhCMB, was purified, characterized and evaluated for immunogenicity and efficacy using multiple adjuvants in a transgenic rodent model. An in vivo TB effect of up to a 65% reduction in intensity and 54% reduction in prevalence was observed using Abisco-100 adjuvant. The ability of this immunogen to induce a TB response was additionally combined with heterologous prime-boost vaccination with viral vectors expressing Pvs25. Significant blockade was observed when combining both platforms, achieving a 74% and 68% reduction in intensity and prevalence, respectively. This observation was confirmed by direct membrane feeding on field P. vivax samples, resulting in reductions in intensity/prevalence of 85.3% and 25.5%. These data demonstrate the potential of this vaccine candidate and support the feasibility of expressing Plasmodium antigens in a plant-based system for the production of TBVs, while demonstrating the potential advantages of combining multiple vaccine delivery systems to maximize efficacy.
AU  - Blagborough,AM
AU  - Musiychuk,K
AU  - Bi,H
AU  - Jones,RM
AU  - Chichester,JA
AU  - Streatfield,S
AU  - Sala,KA
AU  - Zakutansky,SE
AU  - Upton,LM
AU  - Sinden,RE
AU  - Brian,I
AU  - Biswas,S
AU  - Sattabonkot,J
AU  - Yusibov,V
DO  - 10.1016/j.vaccine.2016.05.007.
EP  - 3259
PY  - 2016///
SN  - 1873-2518
SP  - 3252
TI  - Transmission blocking potency and immunogenicity of a plant-produced Pvs25-based subunit vaccine against Plasmodium vivax
T2  - Vaccine
UR  - http://dx.doi.org/10.1016/j.vaccine.2016.05.007.
UR  - http://hdl.handle.net/10044/1/32645
VL  - 34
ER  -
Download