Imperial College London
Portrait Picture

Professor Richard Templer

Faculty of Natural Sciences, Department of Chemistry

Senior Research Investigator
 
 
 
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Contact

 

r.templer Website

 
 
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Assistant

 

Miss Rhea Samra +44 (0)20 7594 8451

 
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Location

 

Electrical EngineeringSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Miller:2016:49/494004,
author = {Miller, DM and Findlay, HE and Ces, O and Templer, RH and Booth, PJ},
doi = {49/494004},
journal = {Nanotechnology},
pages = {1--10},
title = {Light-activated control of protein channel assembly mediated by membrane mechanics},
url = {http://dx.doi.org/10.1088/0957-4484/27/49/494004},
volume = {27},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Photochemical processes provide versatile triggers of chemical reactions. Here, we use a photoactivated lipid switch to modulate the folding and assembly of a protein channel within a model biological membrane. In contrast to the information rich field of water-soluble protein folding, there is only a limited understanding of the assembly of proteins that are integral to biological membranes. It is however possible to exploit the foreboding hydrophobic lipid environment and control membrane protein folding via lipid bilayer mechanics. Mechanical properties such as lipid chain lateral pressure influence the insertion and folding of proteins in membranes, with different stages of folding having contrasting sensitivities to the bilayer properties. Studies to date have relied on altering bilayer properties through lipid compositional changes made at equilibrium, and thus can only be made before or after folding. We show that light-activation of photoisomerisable di-(5-[[4-(4-butylphenyl)azo]phenoxy]pentyl)phosphate (4-Azo-5P) lipids influences the folding and assembly of the pentameric bacterial mechanosensitive channel MscL. The use of a photochemical reaction enables the bilayer properties to be altered during folding, which is unprecedented. This mechanical manipulation during folding, allows for optimisation of different stages of the component insertion, folding and assembly steps within the same lipid system. The photochemical approach offers the potential to control channel assembly when generating synthetic devices that exploit the mechanosensitive protein as a nanovalve.
AU  - Miller,DM
AU  - Findlay,HE
AU  - Ces,O
AU  - Templer,RH
AU  - Booth,PJ
DO  - 49/494004
EP  - 10
PY  - 2016///
SN  - 1361-6528
SP  - 1
TI  - Light-activated control of protein channel assembly mediated by membrane mechanics
T2  - Nanotechnology
UR  - http://dx.doi.org/10.1088/0957-4484/27/49/494004
UR  - http://www.ncbi.nlm.nih.gov/pubmed/27831930
UR  - https://iopscience.iop.org/article/10.1088/0957-4484/27/49/494004
UR  - http://hdl.handle.net/10044/1/43127
VL  - 27
ER  -
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