Imperial College London
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DrRichard Janvan Arkel

Faculty of Engineering, Department of Mechanical Engineering

Senior Lecturer
 
 
 
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Contact

 

+44 (0)20 7594 6157r.vanarkel

 
 
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Location

 

719City and Guilds BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kohli:2023:10.3389/fbioe.2023.1054391,
author = {Kohli, N and Theodoridis, K and Hall, T and Sanz-Pena, I and Gaboriau, D and van, Arkel RJ},
doi = {10.3389/fbioe.2023.1054391},
journal = {Frontiers in Bioengineering and Biotechnology},
pages = {1--12},
title = {Bioreactor analyses of tissue ingrowth, ongrowth and remodelling around implants: an alternative to live animal testing},
url = {http://dx.doi.org/10.3389/fbioe.2023.1054391},
volume = {11},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Introduction: Preclinical assessment of bone remodelling onto, into or around novel implant technologies is underpinned by a large live animal testing burden. The aim of this study was to explore whether a lab-based bioreactor model could provide similar insight.Method: Twelve ex vivo trabecular bone cylinders were extracted from porcine femora and were implanted with additively manufactured stochastic porous titanium implants. Half were cultured dynamically, in a bioreactor with continuous fluid flow and daily cyclic loading, and half in static well plates. Tissue ongrowth, ingrowth and remodelling around the implants were evaluated with imaging and mechanical testing.Results: For both culture conditions, scanning electron microscopy (SEM) revealed bone ongrowth; widefield, backscatter SEM, micro computed tomography scanning, and histology revealed mineralisation inside the implant pores; and histology revealed woven bone formation and bone resorption around the implant. The imaging evidence of this tissue ongrowth, ingrowth and remodelling around the implant was greater for the dynamically cultured samples, and the mechanical testing revealed that the dynamically cultured samples had approximately three times greater push-through fixation strength (p < 0.05).Discussion: Ex vivo bone models enable the analysis of tissue remodelling onto, into and around porous implants in the lab. While static culture conditions exhibited some characteristics of bony adaptation to implantation, simulating physiological conditions with a bioreactor led to an accelerated response.
AU  - Kohli,N
AU  - Theodoridis,K
AU  - Hall,T
AU  - Sanz-Pena,I
AU  - Gaboriau,D
AU  - van,Arkel RJ
DO  - 10.3389/fbioe.2023.1054391
EP  - 12
PY  - 2023///
SN  - 2296-4185
SP  - 1
TI  - Bioreactor analyses of tissue ingrowth, ongrowth and remodelling around implants: an alternative to live animal testing
T2  - Frontiers in Bioengineering and Biotechnology
UR  - http://dx.doi.org/10.3389/fbioe.2023.1054391
UR  - https://www.frontiersin.org/articles/10.3389/fbioe.2023.1054391/full
UR  - http://hdl.handle.net/10044/1/102951
VL  - 11
ER  -
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