Publications

BibTex format

@article{Nottebaum:2021:10.3389/fmolb.2021.669314,
author = {Nottebaum, S and Weinzierl, R},
doi = {10.3389/fmolb.2021.669314},
journal = {Frontiers in Molecular Biosciences},
title = {Transcribing  genes the hard way: in vitro reconstitution of nanoarchaeal RNA polymerase reveals unusual active site properties},
url = {http://dx.doi.org/10.3389/fmolb.2021.669314},
volume = {8},
year = {2021}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Nanoarchaea represent a highly diverged archaeal phylum that displays many unusual biological features. The Nanoarchaeum equitans genome encodes a complete set of RNA polymerase (RNAP) subunits and basal factors. Several of the standard motifs in the active center contain radical substitutions that are normally expected to render the polymerase catalytically inactive. Here we show that, despite these unusual features, a RNAP reconstituted from recombinant Nanoarchaeum subunits is transcriptionally active. Using a sparse-matrix high-throughput screening method we identified an atypical stringent requirement for fluoride ions to maximize its activity under in vitro transcription conditions.
AU  - Nottebaum,S
AU  - Weinzierl,R
DO  - 10.3389/fmolb.2021.669314
PY  - 2021///
SN  - 2296-889X
TI  - Transcribing  genes the hard way: in vitro reconstitution of nanoarchaeal RNA polymerase reveals unusual active site properties
T2  - Frontiers in Molecular Biosciences
UR  - http://dx.doi.org/10.3389/fmolb.2021.669314
UR  - http://hdl.handle.net/10044/1/89804
VL  - 8
ER  -

Download

DrRobertWeinzierl

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)