Publications
110 results found
DIMITRY ES, OSKARSSON T, CONAGHAN J, et al., 1991, BENEFICIAL-EFFECTS OF A 24-H DELAY IN HUMAN CHORIONIC-GONADOTROPIN ADMINISTRATION DURING INVITRO FERTILIZATION TREATMENT CYCLES, HUMAN REPRODUCTION, Vol: 6, Pages: 944-946, ISSN: 0268-1161
- Author Web Link
- Cite
- Citations: 23
WINSTON RML, MARGARA RA, 1991, MICROSURGICAL SALPINGOSTOMY IS NOT AN OBSOLETE PROCEDURE, BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 98, Pages: 637-642, ISSN: 0306-5456
- Author Web Link
- Cite
- Citations: 60
DIMITRY ES, BATES SA, OSKARSSON T, et al., 1991, PROGRAMMING INVITRO FERTILIZATION FOR A 5-DAY OR 3-DAY WEEK, FERTILITY AND STERILITY, Vol: 55, Pages: 934-938, ISSN: 0015-0282
- Author Web Link
- Cite
- Citations: 22
Oskarsson T, Dimitry ES, Mills MS, et al., 1991, Attitudes towards gamete donation among couples undergoing in vitro fertilization., Br J Obstet Gynaecol, Vol: 98, Pages: 351-356, ISSN: 0306-5456
The attitudes of 234 anonymous couples undergoing in vitro fertilization toward sperm and oocyte donation were explored by questionnaire. All the questionnaires were returned of which 222 (95%) were complete and analysed. A high proportion of couples found the use of donor sperm acceptable for therapeutic, diagnostic and treatment purposes (77%, 90% and 97% respectively) and 72%, 84% and 90% respectively were willing to donate oocytes for these purposes. Of potential oocyte donors 41% would agree to nonanonymous donation, 12% would wish to meet the recipient couple and although only 4% wanted to choose the recipient, a quarter of the couples would prefer a relative or friend as the recipient. Provision of nonidentifying information about the donor to the recipient couple was acceptable to almost 70% whereas 40% found giving the same information to the child acceptable.
MASON HD, MARGARA R, WINSTON RML, et al., 1990, INHIBITION OF ESTRADIOL PRODUCTION BY EPIDERMAL GROWTH-FACTOR IN HUMAN GRANULOSA-CELLS OF NORMAL AND POLYCYSTIC OVARIES, CLINICAL ENDOCRINOLOGY, Vol: 33, Pages: 511-517, ISSN: 0300-0664
- Author Web Link
- Cite
- Citations: 77
Hardy K, Martin KL, Leese HJ, et al., 1990, Human preimplantation development in vitro is not adversely affected by biopsy at the 8-cell stage., Hum Reprod, Vol: 5, Pages: 708-714, ISSN: 0268-1161
Normally fertilized human embryos biopsied 3 days after in-vitro fertilization (IVF) have been examined for effects on viability and development in vitro after removal of one or two cells at the 8-cell stage (1/8 and 2/8) from each embryo. A high proportion of 7/8 and 6/8 biopsied and unmanipulated embryos developed to the blastocyst stage between days 5 and 6 (79, 71 and 59%, respectively), and many biopsied embryos (56%) hatched from the zona pellucida in vitro. The viability of biopsied embryos which developed to the blastocyst stage was assessed by daily non-invasive measurement of the uptake of two energy substrates, glucose and pyruvate. Uptake of both substrates was generally lower in 7/8 and 6/8 biopsied embryos but only in proportion to the reduced cellular mass. The total cell number and the numbers of both trophectoderm (TE) and inner cell mass (ICM) cells in biopsied embryos at the blastocyst stage, counted by differential labelling of their nuclei, were also reduced in proportion but the ratio of ICM to TE cells was maintained in both 7/8 and 6/8 biopsied embryos. We conclude that removal of one or two cells at the 8-cell stage, while reducing the cellular mass, does not adversely affect the preimplantation/development of biopsied embryos in vitro and suggest that this approach could be used for preimplantation diagnosis of genetic defects.
HANDYSIDE AH, KONTOGIANNI EH, HARDY K, et al., 1990, PREGNANCIES FROM BIOPSIED HUMAN PREIMPLANTATION EMBRYOS SEXED BY Y-SPECIFIC DNA AMPLIFICATION, NATURE, Vol: 344, Pages: 768-770, ISSN: 0028-0836
- Author Web Link
- Cite
- Citations: 1091
DIMITRY ES, SUBAKSHARPE R, MILLS M, et al., 1990, 9 CASES OF HETEROTOPIC PREGNANCIES IN 4 YEARS OF INVITRO FERTILIZATION, FERTILITY AND STERILITY, Vol: 53, Pages: 107-110, ISSN: 0015-0282
- Author Web Link
- Cite
- Citations: 75
Hardy K, Handyside AH, Winston RM, 1989, The human blastocyst: cell number, death and allocation during late preimplantation development in vitro., Development, Vol: 107, Pages: 597-604, ISSN: 0950-1991
The development of 181 surplus human embryos, including both normally and abnormally fertilized, was observed from day 2 to day 5, 6 or 7 in vitro. 63/149 (42%) normally fertilized embryos reached the blastocyst stage on day 5 or 6. Total, trophectoderm (TE) and inner cell mass (ICM) cell numbers were analyzed by differential labelling of the nuclei with polynucleotide-specific fluorochromes. The TE nuclei were labelled with one fluorochrome during immunosurgical lysis, before fixing the embryo and labelling both sets of nuclei with a second fluorochrome (Handyside and Hunter, 1984, 1986). Newly expanded normally fertilized blastocysts on day 5 had a total of 58.3 +/- 8.1 cells, which increased to 84.4 +/- 5.7 and 125.5 +/- 19 on days 6 and 7, respectively. The numbers of TE cells were similar on days 5 and 6 (37.9 +/- 6.0 and 40.3 +/- 5.0, respectively) and then doubled on day 7 (80.6 +/- 15.2). In contrast, ICM cell numbers doubled between days 5 and 6 (20.4 +/- 4.0 and 41.9 +/- 5.0, respectively) and remained virtually unchanged on day 7 (45.6 +/- 10.2). There was widespread cell death in both the TE and ICM as evidenced by fragmenting nuclei, which increased substantially by day 7. These results are compared with the numbers of cells in morphologically abnormal blastocysts and blastocysts derived from abnormally fertilized embryos. The nuclei of arrested embryos were also examined. The number of TE and ICM cells allocated in normally fertilized blastocysts appears to be similar to the numbers allocated in the mouse. Unlike the mouse, however, the proportion of ICM cells remains higher, despite cell death in both lineages.
Penketh RJ, Delhanty JD, van den Berghe JA, et al., 1989, Rapid sexing of human embryos by non-radioactive in situ hybridization: potential for preimplantation diagnosis of X-linked disorders., Prenat Diagn, Vol: 9, Pages: 489-499, ISSN: 0197-3851
Sixty spare human embryos at various stages of preimplantation development were prepared for cytogenetic analysis. Fluorescent staining of those with metaphases allowed scoring for the presence of a Y chromosome. In situ hybridization was then performed using a biotinylated Y-specific sequence, and the probe was detected by a standard streptavidin-linked alkaline phosphatase system. This enabled comparison of the chromosomal sex with that obtained after in situ hybridization in 28 embryos, and the sexing result obtained by the two methods was concordant in all cases. A further 21 embryos in which no metaphase chromosomes were obtained were sexed by biotinylated in situ hybridization only. Overall, 66 per cent of male interphase nuclei demonstrated a Y-specific hybridization signal. Results were obtained in under 24 h, which may permit the sexing of an embryo biopsied during cleavage and the transfer of sexed embryos at the blastocyst stage to the mother's uterus in the same cycle as oocytes are collected for in vitro fertilization.
POLSON DW, MASON HD, KIDDY DS, et al., 1989, LOW-DOSE FOLLICLE-STIMULATING-HORMONE IN THE TREATMENT OF POLYCYSTIC OVARY SYNDROME - A COMPARISON OF PULSATILE SUBCUTANEOUS WITH DAILY INTRAMUSCULAR THERAPY, BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, Vol: 96, Pages: 746-748, ISSN: 0306-5456
- Author Web Link
- Cite
- Citations: 22
Handyside AH, Pattinson JK, Penketh RJ, et al., 1989, Biopsy of human preimplantation embryos and sexing by DNA amplification., Lancet, Vol: 1, Pages: 347-349, ISSN: 0140-6736
A single cell was removed, through a hole made in the zona pellucida, from each of 30 human embryos at the 6-10 cell cleavage stage three days after in-vitro fertilisation. A normal proportion of the embryos (37%) developed to the blastocyst stage by day six in culture and 6 hatched from the zona. Each male embryo was sexed from the DNA by amplification of a repeated sequence specific for the Y chromosome. In 15 embryos with the normal two pronuclei the sex was determined also by in-situ hybridisation with a Y specific probe or fluorescent chromosome staining to detect metaphase Y chromosomes; the results of Y specific amplification were confirmed. This approach may be valuable for couples at risk of transmitting X-linked disease.
Hardy K, Hooper MA, Handyside AH, et al., 1989, Non-invasive measurement of glucose and pyruvate uptake by individual human oocytes and preimplantation embryos., Hum Reprod, Vol: 4, Pages: 188-191, ISSN: 0268-1161
Pyruvate and glucose uptake by 73 individual human oocytes and preimplantation embryos was measured non-invasively, using an ultramicrofluorescence assay to analyse changes in substrate levels in microdroplets of culture medium. The uptake of both substrates was measured over successive daily incubations between days 1 (unfertilized oocytes) or 2 ('spare' embryos which were not transferred) and day 6 (day 0 = day of insemination). Under these conditions, 58% (25/43) of fertilized embryos with two pronuclei on day 1 developed to the blastocyst stage by day 6. The pyruvate uptake of these embryos increased from approximately 28 to a maximum of 40 pmol/embryo/h between days 2.5 and 4.5. Similarly, glucose uptake increased from approximately 8 to 14 pmol/embryo/h between days 2.5 and 4.5, but then increased further to 24 pmol/embryo/h on day 5 at the blastocyst stage. [corrected] The pyruvate uptake of fertilized embryos which arrested at cleavage stages was significantly lower than for those which developed to the blastocyst stage. Polyspermic and parthenogenetic embryos, and unfertilized oocytes also had lower pyruvate uptakes at later stages. The glucose uptake of unfertilized oocytes and abnormal embryos never reached the level of fertilized embryos at the blastocyst stage on day 5.5. Non-invasive measurement of pyruvate uptake before embryo transfer may provide a valuable functional criterion for the selection of viable embryos capable of developing to the blastocyst stage.
RUTHERFORD AJ, SUBAKSHARPE RJ, DAWSON KJ, et al., 1988, IMPROVEMENT OF INVITRO FERTILIZATION AFTER TREATMENT WITH BUSERELIN, AN AGONIST OF LUTEINIZING-HORMONE RELEASING HORMONE, BRITISH MEDICAL JOURNAL, Vol: 296, Pages: 1765-1768, ISSN: 0959-8138
- Author Web Link
- Cite
- Citations: 150
FRANKS S, MASON HD, POLSON DW, et al., 1988, MECHANISM AND MANAGEMENT OF OVULATORY FAILURE IN WOMEN WITH POLYCYSTIC OVARY SYNDROME, HUMAN REPRODUCTION, Vol: 3, Pages: 531-534, ISSN: 0268-1161
- Author Web Link
- Cite
- Citations: 69
HARLOW CR, WINSTON RML, MARGARA RA, et al., 1987, GONADOTROPIC CONTROL OF HUMAN GRANULOSA-CELL GLYCOLYSIS, HUMAN REPRODUCTION, Vol: 2, Pages: 649-653, ISSN: 0268-1161
- Author Web Link
- Cite
- Citations: 36
WINSTON R, MARGARA R, 1987, EFFECTIVENESS OF TREATMENT FOR INFERTILITY, BRITISH MEDICAL JOURNAL, Vol: 295, Pages: 608-609, ISSN: 0959-8138
- Author Web Link
- Cite
- Citations: 4
Otubu JA, Winston RM, Wineman M, et al., 1987, Morphology of human and experimental hydrosalpinges: a comparative study., Afr J Med Med Sci, Vol: 16, Pages: 79-88, ISSN: 0309-3913
Infection, and possibly raised intra-luminal pressure, may be responsible for the morphological changes and associated infertility that occur with hydrosalpinx formation. Biopsies from human hydrosalpinges were studied by light microscopy and scanning and transmission electron microscopy. Loss of mucosal folds, atrophied epithelium and varying degrees of deciliation were the main features found. Mechanical hydrosalpinges were produced in rabbits by double clip application (duration of clip application varied from 8 to 52 weeks). Morphological study of animal hydrosalpinges showed partial or complete loss of mucosal folds, atrophied epithelium and varying degrees of deciliation. The severity of morphological changes was related to duration of clip application. It is concluded that raised intra-luminal pressure plays a role in the morphological changes seen in human hydrosalpinges and infertility in women after anatomically successful salpingostomy. The rabbit would appear to provide a suitable model for the study of human hydrosalpinges. The significance of these findings is discussed in relation to the clinical management of hydrosalpinges.
WINSTON R, 1987, CLINICAL ALGORITHMS - INFERTILITY, BRITISH MEDICAL JOURNAL, Vol: 294, Pages: 1229-1230, ISSN: 0959-8138
WINSTON R, HILLIER S, 1985, IVF VERSUS NATURE, LANCET, Vol: 1, Pages: 284-284, ISSN: 0140-6736
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.