Imperial College London
Professor Lord Robert Winston

Professor LordRobertWinston

Central Faculty, Division of the University Secretary

Professor of Science and Society
 
 
 
//

Contact

 

+44 (0)20 7594 9363r.winston Website

 
 
//

Assistant

 

Ms Helen Wadsworth +44 (0)20 7594 5959

 
//

Location

 

205Skempton BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Carlsson:2006:10.1530/rep.1.00868,
author = {Carlsson, IB and Laitinen, MPE and Scott, JE and Louhio, H and Velentzis, L and Tuuri, T and Aaltonen, J and Ritvos, O and Winston, RML and Hovatta, O},
doi = {10.1530/rep.1.00868},
journal = {Reproduction},
pages = {641--649},
title = {Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture.},
url = {http://dx.doi.org/10.1530/rep.1.00868},
volume = {131},
year = {2006}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The receptor tyrosine c-Kit and its cognate ligand, c-Kit ligand (KL, stem cell factor, SCF), are involved in ovarian follicular development in several animal species. We studied the expression of KL and c-Kit using in situ hybridization and immunohistochemistry in donated human ovarian cortical tissue. The KL transcripts were expressed in granulosa cells of primary follicles, whereas the expression of c-Kit was confined to the oocyte and granulosa cells in primary and secondary follicles. We employed an ovarian organ culture using firstly serum-containing and then serum-free medium to study the effects of KL and an anti-c-Kit antibody, ACK2, on the development and survival of ovarian follicles in vitro. Culture of ovarian cortical slices for 7 days resulted in a 37% increase in the number of primary follicles and a 6% increase in secondary follicles. The proportion of viable follicles decreased in all cultures. The addition of KL (1, 10 and 100 ng/ml) into the culture media did not affect the developmental stages of the follicles or the proportion of atretic follicles. Inclusion of ACK2 (800 ng/ml) in the culture medium significantly increased the proportion of atretic follicles on days 7 (49 vs 28% in control cultures) and 14 (62 vs 38%) of culture. In conclusion, c-Kit and KL are expressed in human ovaries during follicular development. Blocking the c-Kit receptor induces follicular atresia. The KL/c-Kit signaling system is likely to control the survival of human ovarian follicles during early follicular development.
AU  - Carlsson,IB
AU  - Laitinen,MPE
AU  - Scott,JE
AU  - Louhio,H
AU  - Velentzis,L
AU  - Tuuri,T
AU  - Aaltonen,J
AU  - Ritvos,O
AU  - Winston,RML
AU  - Hovatta,O
DO  - 10.1530/rep.1.00868
EP  - 649
PY  - 2006///
SN  - 1470-1626
SP  - 641
TI  - Kit ligand and c-Kit are expressed during early human ovarian follicular development and their interaction is required for the survival of follicles in long-term culture.
T2  - Reproduction
UR  - http://dx.doi.org/10.1530/rep.1.00868
UR  - https://www.ncbi.nlm.nih.gov/pubmed/16595715
VL  - 131
ER  -
Download