Imperial College London
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Dr Woscholski

Faculty of Natural Sciences, Department of Chemistry

Reader in Chemical Biology
 
 
 
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Contact

 

+44 (0)20 7594 5305r.woscholski

 
 
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Location

 

301LMolecular Sciences Research HubWhite City Campus

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Summary

 

Publications

Citation

BibTex format

@article{Domart:2012:10.1371/journal.pone.0051150,
author = {Domart, M-C and Hobday, TMC and Peddie, CJ and Chung, GHC and Wang, A and Yeh, K and Jethwa, N and Zhang, Q and Wakelam, MJO and Woscholski, R and Byrne, RD and Collinson, LM and Poccia, DL and Larijani, B},
doi = {10.1371/journal.pone.0051150},
journal = {PLoS ONE},
title = {Acute manipulation of diacylglycerol reveals roles in nuclear envelope assembly & endoplasmic reticulum morphology},
url = {http://dx.doi.org/10.1371/journal.pone.0051150},
volume = {7},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The functions and morphology of cellular membranes are intimately related and depend not only on their protein content but also on the repertoire of lipids that comprise them. In the absence of in vivo data on lipid asymmetry in endomembranes, it has been argued that motors, scaffolding proteins or integral membrane proteins rather than non-lamellar bilayer lipids such as diacylglycerol (DAG), are responsible for shaping of organelles, local membrane curvature and fusion. The effects of direct alteration of levels of such lipids remain predominantly uninvestigated. Diacylglycerol (DAG) is a well documented second messenger. Here we demonstrate two additional conserved functions of DAG: a structural role in organelle morphology, and a role in localised extreme membrane curvature required for fusion for which proteins alone are insufficient. Acute and inducible DAG depletion results in failure of the nuclear envelope (NE) to reform at mitosis and reorganisation of the ER into multi-lamellar sheets as revealed by correlative light and electron microscopy and 3D reconstructions. Remarkably, depleted cells divide without a complete NE, and unless rescued by 1,2 or 1,3 DAG soon die. Attenuation of DAG levels by enzyme microinjection into echinoderm eggs and embryos also results in alterations of ER morphology and nuclear membrane fusion. Our findings demonstrate that DAG is an in vivo modulator of organelle morphology in mammalian and echinoderm cells, indicating a fundamental role conserved across the deuterostome superphylum.
AU  - Domart,M-C
AU  - Hobday,TMC
AU  - Peddie,CJ
AU  - Chung,GHC
AU  - Wang,A
AU  - Yeh,K
AU  - Jethwa,N
AU  - Zhang,Q
AU  - Wakelam,MJO
AU  - Woscholski,R
AU  - Byrne,RD
AU  - Collinson,LM
AU  - Poccia,DL
AU  - Larijani,B
DO  - 10.1371/journal.pone.0051150
PY  - 2012///
SN  - 1932-6203
TI  - Acute manipulation of diacylglycerol reveals roles in nuclear envelope assembly & endoplasmic reticulum morphology
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0051150
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000312588200106&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/67858
VL  - 7
ER  -
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