Imperial College London
Dr Ronald Pearce

DrRonaldPearce

Faculty of Medicine, Faculty of Medicine Centre

Honorary Clinical Senior Lecturer
 
 
 
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Contact

 

ronald.pearce

 
 
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Assistant

 

Dr Stavia Blunt +44 (0)20 3311 1182

 
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Location

 

10L15ACharing Cross HospitalCharing Cross Campus

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Summary

 

Publications

Citation

BibTex format

@article{Liu:2015:10.1111/nan.12293,
author = {Liu, AKL and Hurry, MED and Ng, OTW and DeFelice, J and Lai, HM and Pearce, RKB and Wong, GTC and Chang, RCC and Gentleman, SM},
doi = {10.1111/nan.12293},
journal = {Neuropathology and Applied Neurobiology},
pages = {573--587},
title = {Bringing CLARITY to the human brain: visualization of Lewy pathology in three dimensions},
url = {http://dx.doi.org/10.1111/nan.12293},
volume = {42},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Aims: CLARITY is a novel technique which enables three-dimensional visualisation of immunostained tissue for the study of circuitry and spatial interactions between cells and molecules in the brain. In this study we aimed to compare methodological differences in the application of CLARITY between rodent and large human post-mortem brain samples. In addition, we aimed to investigate if this technique could be used to visualise Lewy pathology in a post-mortem Parkinson’s brain. Methods: Rodent and human brain samples were clarified and immunostained using the passive version of the CLARITY technique. Samples were then immersed in different refractive index matching media before mounting and visualising under a confocal microscope. Results:  We found that tissue clearing speed using passive CLARITY differs according to species (human vs rodents), brain region and degree of fixation (fresh vs formalin-fixed tissues). Furthermore, there were advantages to using specific refractive index matching media. We have applied this technique and have successfully visualised Lewy body inclusions in three dimensions within the nucleus basalis of Meynert, and the spatial relationship between monoaminergic fibres and Lewy pathologies among nigrostriatal fibres in the midbrain without the need for physical serial sectioning of brain tissue. Conclusions: The effective use of CLARITY on large samples of human tissue opens up many potential avenues for detailed pathological and morphological studies.
AU  - Liu,AKL
AU  - Hurry,MED
AU  - Ng,OTW
AU  - DeFelice,J
AU  - Lai,HM
AU  - Pearce,RKB
AU  - Wong,GTC
AU  - Chang,RCC
AU  - Gentleman,SM
DO  - 10.1111/nan.12293
EP  - 587
PY  - 2015///
SN  - 1365-2990
SP  - 573
TI  - Bringing CLARITY to the human brain: visualization of Lewy pathology in three dimensions
T2  - Neuropathology and Applied Neurobiology
UR  - http://dx.doi.org/10.1111/nan.12293
UR  - http://hdl.handle.net/10044/1/28368
VL  - 42
ER  -
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