Imperial College London
Alternate

ProfessorRongjunChen

Faculty of Engineering, Department of Chemical Engineering

Professor of Biomaterials Engineering
 
 
 
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Contact

 

+44 (0)20 7594 2070rongjun.chen Website

 
 
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Location

 

408ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Chen:2020:10.1021/acsami.0c03260,
author = {Chen, S and Wu, L and Ren, J and Bemmer, VL and Zajicek, R and Chen, R},
doi = {10.1021/acsami.0c03260},
journal = {ACS Applied Materials & Interfaces},
pages = {28941--28951},
title = {Comb-like pseudopeptides enable very rapid and efficient intracellular trehalose delivery for enhanced cryopreservation of erythrocytes},
url = {http://dx.doi.org/10.1021/acsami.0c03260},
volume = {12},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cell cryopreservation plays a key role in development of reproducible and cost-effective cell-based therapies. Trehalose accumulated in freezing and desiccation tolerant organisms in nature has been sought as an attractive non-toxic cryoprotectant. Herein, we report a co-incubation method for very rapid and efficient delivery of membrane-impermeable trehalose into ovine erythrocytes through reversible membrane permeabilization using pH-responsive, comb-like pseudopeptides. The pseudopeptidic polymers containing relatively long alkyl side chains were synthesized to mimic membrane-anchoring fusogenic proteins. The intracellular trehalose delivery efficiency was optimized by manipulating the side chain length, degree of substitution and concentration of the pseudopeptides with different hydrophobic alkyl side chains, the pH, temperature and time of incubation, as well as the polymer-to-cell ratio and the concentration of extracellular trehalose. Treatment of erythrocytes with the comb-like pseudopeptides for only 15 min yielded an intracellular trehalose concentration of 177.9 ± 8.6 mM, which resulted in 90.3 ± 0.7% survival after freeze-thaw. The very rapid and efficient delivery was found to be attributed to the reversible, pronounced membrane curvature change as a result of strong membrane insertion of the comb-like pseudopeptides. The pseudopeptides can enable efficient intracellular delivery of not only trehalose for improved cell cryopreservation, but also other membrane-impermeable cargos.
AU  - Chen,S
AU  - Wu,L
AU  - Ren,J
AU  - Bemmer,VL
AU  - Zajicek,R
AU  - Chen,R
DO  - 10.1021/acsami.0c03260
EP  - 28951
PY  - 2020///
SN  - 1944-8244
SP  - 28941
TI  - Comb-like pseudopeptides enable very rapid and efficient intracellular trehalose delivery for enhanced cryopreservation of erythrocytes
T2  - ACS Applied Materials & Interfaces
UR  - http://dx.doi.org/10.1021/acsami.0c03260
UR  - https://pubs.acs.org/doi/10.1021/acsami.0c03260
UR  - http://hdl.handle.net/10044/1/79930
VL  - 12
ER  -
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