Imperial College London
Alternate

ProfessorRongjunChen

Faculty of Engineering, Department of Chemical Engineering

Professor of Biomaterials Engineering
 
 
 
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Contact

 

+44 (0)20 7594 2070rongjun.chen Website

 
 
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Location

 

408ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Tan:2021:10.1016/j.biomaterials.2021.121061,
author = {Tan, P and Cai, H and Wei, Q and Tang, X and Zhang, Q and Kopytynski, M and Yang, J and Yi, Y and Zhang, H and Gong, Q and Gu, Z and Chen, R and Luo, K},
doi = {10.1016/j.biomaterials.2021.121061},
journal = {Biomaterials},
pages = {1--13},
title = {Enhanced chemo-photodynamic therapy of an enzyme-responsive prodrug in bladder cancer patient-derived xenograft models},
url = {http://dx.doi.org/10.1016/j.biomaterials.2021.121061},
volume = {277},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Patient-derived xenograft (PDX) models are powerful tools for understanding cancer biology and drug discovery. In this study, a polymeric nano-sized drug delivery system poly(OEGMA)-PTX@Ce6 (NPs@Ce6) composed of a photosensitizer chlorin e6 (Ce6) and a cathepsin B-sensitive polymer-paclitaxel (PTX) prodrug was constructed. The photochemical internalization (PCI) effect and enhanced chemo-photodynamic therapy (PDT) were achieved via a two-stage light irradiation strategy. The results showed that the NPs@Ce6 had great tumor targeting and rapid cellular uptake induced by PCI, thereby producing excellent anti-tumor effects on human bladder cancer PDX models with tumor growth inhibition greater than 98%. Bioinformatics analysis revealed that the combination of PTX chemotherapy and PDT up-regulated oxidative phosphorylation and ROS generation, blocked cell cycle and proliferation, and down-regulated the pathways related to tumor progression, invasion and metastasis, including hypoxia, TGF-β signaling and TNF-α signaling pathways. Western blots analysis confirmed that proteins promoting apoptosis (Bax, cleaved caspase-3, cleaved PARP) and DNA damage (γH2A.X) were up-regulated, while those inhibiting apoptosis (Bcl-2) and mitosis (pan-actin and α/β-tubulin) were down-regulated after chemo-PDT treatment. Therefore, this stimuli-responsive polymer-PTX prodrug-based nanomedicine with combinational chemotherapy and PDT evaluated in the PDX models could be a potential candidate for bladder cancer therapy.
AU  - Tan,P
AU  - Cai,H
AU  - Wei,Q
AU  - Tang,X
AU  - Zhang,Q
AU  - Kopytynski,M
AU  - Yang,J
AU  - Yi,Y
AU  - Zhang,H
AU  - Gong,Q
AU  - Gu,Z
AU  - Chen,R
AU  - Luo,K
DO  - 10.1016/j.biomaterials.2021.121061
EP  - 13
PY  - 2021///
SN  - 0142-9612
SP  - 1
TI  - Enhanced chemo-photodynamic therapy of an enzyme-responsive prodrug in bladder cancer patient-derived xenograft models
T2  - Biomaterials
UR  - http://dx.doi.org/10.1016/j.biomaterials.2021.121061
UR  - https://www.sciencedirect.com/science/article/pii/S0142961221004178?via%3Dihub
UR  - http://hdl.handle.net/10044/1/91342
VL  - 277
ER  -
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