Imperial College London
Alternate

ProfessorRongjunChen

Faculty of Engineering, Department of Chemical Engineering

Professor of Biomaterials Engineering
 
 
 
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Contact

 

+44 (0)20 7594 2070rongjun.chen Website

 
 
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Location

 

408ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Yan:2023:10.1101/2023.02.07.524134,
author = {Yan, D and Lu, H and Kaur, A and Fu, R and Wang, N and Teh, JH and Lou, H and Aboagye, E and Chen, R},
doi = {10.1101/2023.02.07.524134},
title = {Development and optimisation of cationic lipid nanoparticles for mRNA delivery},
url = {http://dx.doi.org/10.1101/2023.02.07.524134},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Messenger RNA (mRNA) has been proposed as a therapeutic agent for various diseases, including cancer. To ensure effective transfection of cancer cells, mRNA needs to be transported with a delivery system that protects its integrity and functionality. In this regard, cationic lipid nanoparticles composed of dioleoylphosphatidylethanolamine (DOPE) and 3β-[N-(N’,N’-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol) have emerged as common vectors to deliver mRNA. In this project, we aim to use luciferase mRNA as a reporter to synthesise mRNA-loaded cationic lipid nanoparticles, and optimise their mRNA encapsulation and transfection efficiency in ovarian cancer cells. The optimisation process included: 1) adjusting the lipid formulation; 2) adjusting the input mRNA concentration before lipid nanoparticle extrusion; and 3) adjusting the extrusion methods. After optimisation, the encapsulation efficiency was optimised to 62%, thus achieving a relatively high transfection luminescence signal (9.4 times compared to baseline). The lipid nanoparticles also demonstrated stable physical characteristics and high biocompatibility (above 75% cell viability after treatment) within 24 hours. Overall, this project evaluated the synthesis of DOPE/DC-Chol cationic lipid nanoparticles, and optimised their mRNA encapsulation and transfection efficiency in ovarian cancer cell lines. The optimised lipid nanoparticles can be utilised as an ideal system for mRNA delivery, which could be further developed as a potential platform for the immunotherapy in ovarian cancer.
AU  - Yan,D
AU  - Lu,H
AU  - Kaur,A
AU  - Fu,R
AU  - Wang,N
AU  - Teh,JH
AU  - Lou,H
AU  - Aboagye,E
AU  - Chen,R
DO  - 10.1101/2023.02.07.524134
PY  - 2023///
TI  - Development and optimisation of cationic lipid nanoparticles for mRNA delivery
UR  - http://dx.doi.org/10.1101/2023.02.07.524134
ER  -
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