Imperial College London
Alternate

ProfessorRongjunChen

Faculty of Engineering, Department of Chemical Engineering

Professor of Biomaterials Engineering
 
 
 
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Contact

 

+44 (0)20 7594 2070rongjun.chen Website

 
 
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Location

 

408ACE ExtensionSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Wang:2017:10.1021/acsami.6b13807,
author = {Wang, W and Guo, Y and Tiede, C and Chen, S and Kopytynski, M and Kong, Y and Kulak, A and Tomlinson, D and Chen, R and McPherson, M and Zhou, D},
doi = {10.1021/acsami.6b13807},
journal = {ACS Applied Materials & Interfaces},
pages = {15232--15244},
title = {Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging.},
url = {http://dx.doi.org/10.1021/acsami.6b13807},
volume = {9},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - An ultraefficient cap-exchange protocol (UCEP) that can convert hydrophobic quantum dots (QDs) into stable, biocompatible, and aggregation-free water-dispersed ones at a ligand:QD molar ratio (LQMR) as low as 500, some 20-200-fold less than most literature methods, has been developed. The UCEP works conveniently with air-stable lipoic acid (LA)-based ligands by exploiting tris(2-carboxylethyl phosphine)-based rapid in situ reduction. The resulting QDs are compact (hydrodynamic radius, Rh, < 4.5 nm) and bright (retaining > 90% of original fluorescence), resist nonspecific adsorption of proteins, and display good stability in biological buffers even with high salt content (e.g., 2 M NaCl). These advantageous properties make them well suited for cellular imaging and ratiometric biosensing applications. The QDs prepared by UCEP using dihydrolipoic acid (DHLA)-zwitterion ligand can be readily conjugated with octa-histidine (His8)-tagged antibody mimetic proteins (known as Affimers). These QDs allow rapid, ratiometric detection of the Affimer target protein down to 10 pM via a QD-sensitized Förster resonance energy transfer (FRET) readout signal. Moreover, compact biotinylated QDs can be readily prepared by UCEP in a facile, one-step process. The resulting QDs have been further employed for ratiometric detection of protein, exemplified by neutravidin, down to 5 pM, as well as for fluorescence imaging of target cancer cells.
AU  - Wang,W
AU  - Guo,Y
AU  - Tiede,C
AU  - Chen,S
AU  - Kopytynski,M
AU  - Kong,Y
AU  - Kulak,A
AU  - Tomlinson,D
AU  - Chen,R
AU  - McPherson,M
AU  - Zhou,D
DO  - 10.1021/acsami.6b13807
EP  - 15244
PY  - 2017///
SN  - 1944-8244
SP  - 15232
TI  - Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging.
T2  - ACS Applied Materials & Interfaces
UR  - http://dx.doi.org/10.1021/acsami.6b13807
UR  - http://www.ncbi.nlm.nih.gov/pubmed/28421739
UR  - http://hdl.handle.net/10044/1/48968
VL  - 9
ER  -
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