Imperial College London
Alternate

Professor Sir Roy Anderson FRS, FMedSci

Faculty of Medicine, School of Public Health

Professor in Infectious Disease Epidemiology
 
 
 
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Contact

 

roy.anderson Website

 
 
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Assistant

 

Mrs Clare Mylchreest +44 (0)7766 331 301

 
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Location

 

LG35Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Easton:2019:10.1128/mBio.00519-19,
author = {Easton, AV and Quiñones, M and Vujkovic-Cvijin, I and Oliveira, RG and Kepha, S and Odiere, MR and Anderson, RM and Belkaid, Y and Nutman, TB},
doi = {10.1128/mBio.00519-19},
journal = {mBio},
title = {The impact of anthelmintic treatment on human gut microbiota based on cross-sectional and pre- and postdeworming comparisons in Western Kenya},
url = {http://dx.doi.org/10.1128/mBio.00519-19},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Murine studies suggest that the presence of some species of intestinal helminths is associated with changes in host microbiota composition and diversity. However, studies in humans have produced varied conclusions, and the impact appears to vary widely depending on the helminth species present. To demonstrate how molecular approaches to the human gut microbiome can provide insights into the complex interplay among disparate organisms, DNA was extracted from cryopreserved stools collected from residents of 5 rural Kenyan villages prior to and 3 weeks and 3 months following albendazole (ALB) therapy. Samples were analyzed by quantitative PCR (qPCR) for the presence of 8 species of intestinal parasites and by MiSeq 16S rRNA gene sequencing. Based on pretreatment results, the presence of neither Ascaris lumbricoides nor Necator americanus infection significantly altered the overall diversity of the microbiota in comparison with age-matched controls. Following ALB therapy and clearance of soil-transmitted helminths (STH), there were significant increases in the proportion of the microbiota made up by Clostridiales (P = 0.0002; average fold change, 0.57) and reductions in the proportion made up by Enterobacteriales (P = 0.0004; average fold change, -0.58). There was a significant posttreatment decrease in Chao1 richness, even among individuals who were uninfected pretreatment, suggesting that antimicrobial effects must be considered in any posttreatment setting. Nevertheless, the helminth-associated changes in Clostridiales and Enterobacteriales suggest that clearance of STH, and of N. americanus in particular, alters the gut microbiota.IMPORTANCE The gut microbiome is an important factor in human health. It is affected by what we eat, what medicines we take, and what infections we acquire. In turn, it affects the way we absorb nutrients and whether we have excessive intestinal inflammation. Intestinal worms may have an important
AU  - Easton,AV
AU  - Quiñones,M
AU  - Vujkovic-Cvijin,I
AU  - Oliveira,RG
AU  - Kepha,S
AU  - Odiere,MR
AU  - Anderson,RM
AU  - Belkaid,Y
AU  - Nutman,TB
DO  - 10.1128/mBio.00519-19
PY  - 2019///
SN  - 2150-7511
TI  - The impact of anthelmintic treatment on human gut microbiota based on cross-sectional and pre- and postdeworming comparisons in Western Kenya
T2  - mBio
UR  - http://dx.doi.org/10.1128/mBio.00519-19
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31015324
UR  - http://hdl.handle.net/10044/1/72029
VL  - 10
ER  -
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