Imperial College London
Alternate

ProfessorRylieGreen

Faculty of Engineering, Department of Bioengineering

Head of the Department of Bioengineering
 
 
 
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Contact

 

+44 (0)20 7594 0943rylie.green

 
 
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Location

 

3.05Bessemer BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Roberts:2016:10.1177/2041731416677132,
author = {Roberts, JJ and Farrugia, BL and Green, RA and Rnjak-Kovacina, J and Martens, PJ},
doi = {10.1177/2041731416677132},
journal = {J Tissue Eng},
title = {In situ formation of poly(vinyl alcohol)-heparin hydrogels for mild encapsulation and prolonged release of basic fibroblast growth factor and vascular endothelial growth factor.},
url = {http://dx.doi.org/10.1177/2041731416677132},
volume = {7},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Heparin-based hydrogels are attractive for controlled growth factor delivery, due to the native ability of heparin to bind and stabilize growth factors. Basic fibroblast growth factor and vascular endothelial growth factor are heparin-binding growth factors that synergistically enhance angiogenesis. Mild, in situ encapsulation of both basic fibroblast growth factor and vascular endothelial growth factor and subsequent bioactive dual release has not been demonstrated from heparin-crosslinked hydrogels, and the combined long-term delivery of both growth factors from biomaterials is still a major challenge. Both basic fibroblast growth factor and vascular endothelial growth factor were encapsulated in poly(vinyl alcohol)-heparin hydrogels and demonstrated controlled release. A model cell line, BaF32, was used to show bioactivity of heparin and basic fibroblast growth factor released from the gels over multiple days. Released basic fibroblast growth factor promoted higher human umbilical vein endothelial cell outgrowth over 24 h and proliferation for 3 days than the poly(vinyl alcohol)-heparin hydrogels alone. The release of vascular endothelial growth factor from poly(vinyl alcohol)-heparin hydrogels promoted human umbilical vein endothelial cell outgrowth but not significant proliferation. Dual-growth factor release of basic fibroblast growth factor and vascular endothelial growth factor from poly(vinyl alcohol)-heparin hydrogels resulted in a synergistic effect with significantly higher human umbilical vein endothelial cell outgrowth compared to basic fibroblast growth factor or vascular endothelial growth factor alone. Poly(vinyl alcohol)-heparin hydrogels allowed bioactive growth factor encapsulation and provided controlled release of multiple growth factors which is beneficial toward tissue regeneration applications.
AU  - Roberts,JJ
AU  - Farrugia,BL
AU  - Green,RA
AU  - Rnjak-Kovacina,J
AU  - Martens,PJ
DO  - 10.1177/2041731416677132
PY  - 2016///
SN  - 2041-7314
TI  - In situ formation of poly(vinyl alcohol)-heparin hydrogels for mild encapsulation and prolonged release of basic fibroblast growth factor and vascular endothelial growth factor.
T2  - J Tissue Eng
UR  - http://dx.doi.org/10.1177/2041731416677132
UR  - https://www.ncbi.nlm.nih.gov/pubmed/27895888
VL  - 7
ER  -
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