14 results found
Wright PT, Sanchez-Alonso JL, Lucarelli C, et al., 2018, Partial mechanical unloading of the heart disrupts L-type calcium channel and beta-adrenoceptor signaling microdomains, Frontiers in Physiology, Vol: 9, ISSN: 1664-042X
Introduction: We investigated the effect of partial mechanical unloading (PMU) of the heart on the physiology of calcium and beta-adrenoceptor-cAMP (βAR-cAMP) microdomains. Previous studies have investigated PMU using a model of heterotopic-heart and lung transplantation (HTHAL). These studies have demonstrated that PMU disrupts the structure of cardiomyocytes and calcium handling. We sought to understand these processes by studying L-Type Calcium Channel (LTCC) activity and sub-type-specific βAR-cAMP signaling within cardiomyocyte membrane microdomains.Method: We utilized an 8-week model of HTHAL, whereby the hearts of syngeneic Lewis rats were transplanted into the abdomens of randomly assigned cage mates. A pronounced atrophy was observed in hearts after HTHAL. Cardiomyocytes were isolated via enzymatic perfusion. We utilized Förster Resonance Energy Transfer (FRET) based cAMP-biosensors and scanning ion conductance microscopy (SICM) based methodologies to study localization of LTCC and βAR-cAMP signaling.Results: β2AR-cAMP responses measured by FRET in the cardiomyocyte cytosol were reduced by PMU (loaded 28.51 ± 7.18% vs. unloaded 10.84 ± 3.27% N,n 4/10-13 mean ± SEM ∗p < 0.05). There was no effect of PMU on β2AR-cAMP signaling in RII_Protein Kinase A domains. β1AR-cAMP was unaffected by PMU in either microdomain. Consistent with this SICM/FRET analysis demonstrated that β2AR-cAMP was specifically reduced in t-tubules (TTs) after PMU (loaded TT 0.721 ± 0.106% vs. loaded crest 0.104 ± 0.062%, unloaded TT 0.112 ± 0.072% vs. unloaded crest 0.219 ± 0.084% N,n 5/6-9 mean ± SEM ∗∗p < 0.01, ∗∗∗p < 0.001 vs. loaded TT). By comparison β1AR-cAMP responses in either TT or sarcolemmal crests were unaffected by the PMU. LTCC occurrence and open probability (Po) were reduced by PMU (loaded TT Po 0.073 ± 0.011% vs. load
Wright P, Lucarelli C, Sanchez-Alonso J, et al., 2018, Mechanical Unloading Suppresses Localized Beta-2 Adrenoceptor and L-type Calcium Channel Function in Healthy and Failing Cardiomyocytes, Circulation 136(Suppl_1.18365):14 Nov 2017
Bello S, Singh C, Jayakumar S, et al., 2018, Role of Cardiomyocyte Regeneration in Mechanical Unloading Induced Reverse Cardiac Remodeling, Circulation 134(Suppl_1.20205):11 Nov 2016, ISSN: 0009-7322
Bello S, Singh C, Punjabi P, et al., 2017, Coronary Reperfusion Ameliorates the Deleterious Effect of Mechanical Unloading on Infarct Size and Interstitial Fibrosis After Acute Myocardial Infarction, Circulation, Vol: 136
Mawad D, Mansfield C, Lauto A, et al., 2016, A conducting polymer with enhanced electronic stability applied in cardiac models, Science Advances, Vol: 2, ISSN: 2375-2548
Electrically active constructs can have a beneficial effect on electroresponsive tissues, such as the brain, heart, and nervous system. Conducting polymers (CPs) are being considered as components of these constructs because of their intrinsic electroactive and flexible nature. However, their clinical application has been largely hampered by their short operational time due to a decrease in their electronic properties. We show that, by immobilizing the dopant in the conductive scaffold, we can prevent its electric deterioration. We grew polyaniline (PANI) doped with phytic acid on the surface of a chitosan film. The strong chelation between phytic acid and chitosan led to a conductive patch with retained electroactivity, low surface resistivity (35.85 ± 9.40 kilohms per square), and oxidized form after 2 weeks of incubation in physiological medium. Ex vivo experiments revealed that the conductive nature of the patch has an immediate effect on the electrophysiology of the heart. Preliminary in vivo experiments showed that the conductive patch does not induce proarrhythmogenic activities in the heart. Our findings set the foundation for the design of electronically stable CP-based scaffolds. This provides a robust conductive system that could be used at the interface with electroresponsive tissue to better understand the interaction and effect of these materials on the electrophysiology of these tissues.
Alassar A, Bello SOZ, Rahamim J, 2015, Novel approach to drainage of a right subphrenic abscess using trans-diaphragmatic laparoscopy, ANNALS OF THE ROYAL COLLEGE OF SURGEONS OF ENGLAND, Vol: 97, Pages: 543-543, ISSN: 0035-8843
Bello SOZ, Rahamim J, 2015, High-Dose Intravenous Octreotide Is Safe and May Be Superior to Surgery in Managing Severe Postesophagectomy Chylothorax in High-Risk Patients, ANNALS OF THORACIC SURGERY, Vol: 100, Pages: 297-299, ISSN: 0003-4975
Terracciano CM, Navaratnarajah M, Bello SOZ, et al., 2015, Does Size Matter? In search of a physiological definition of myocardial atrophy, JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY, Vol: 65, Pages: 2154-2156, ISSN: 0735-1097
Bello SOZ, Page A, Sadat U, et al., 2013, Chest X-ray and electrocardiogram in post-cardiac surgery follow-up clinics: should this be offered routinely or when clinically indicated?, INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY, Vol: 16, Pages: 725-730, ISSN: 1569-9293
Sivarajah V, Bello SOZ, Yiu CY, et al., 2011, Gluteo-vaginal fistula: a long-term complication of posterior intravaginal slingplasty., BMJ Case Rep, Vol: 2011
A 67-year-old lady presented to the surgical outpatient clinic with a 4 month history of recurrent purulent discharge from her left buttock. Four years and 4 months prior to this she underwent a posterior intravaginal slingoplasty for vaginal prolapse and urinary stress incontinence. An MRI demonstrated a long gluteo-vaginal fistula tract from the posterior wall of the vaginal vault through the left ischiorectal fossa to the skin. An examination under anaesthesia revealed that the fistulous tract was surrounding the intact mesh used for the posterior intravaginal slingoplasty. The mesh was removed, the fistula tract excised and the perineal wound marsupialised. The patient was discharged 5 days later. The wound healed within 4 weeks and she remains sepsis free 2 years on.
Bello SOZ, Peng EWK, Sarkar PK, 2011, Conduits for coronary artery bypass surgery: the quest for second best, JOURNAL OF CARDIOVASCULAR MEDICINE, Vol: 12, Pages: 411-421, ISSN: 1558-2027
Bello SOZ, Kouerinis I, Pillay W, 2011, Spontaneous supraceliac isolated abdominal aortic dissection sparing major visceral and renal vessels and presenting as chronic limb ischemia., Int J Vasc Med, Vol: 2011
Aortic dissections that originate from isolated tears in the abdominal aorta are uncommon. Rarer still are cases of isolated abdominal aortic dissections arising in suprarenal locations, as most appear from infrarenal intimal defects. We present a quite unusual case of a spontaneous supraceliac isolated abdominal aortic dissection sparing the renal and mesenteric arteries and manifesting as chronic rather than acute limb ischemia. The atypical presentation of this case led to repeated misdiagnosis with consequent loss of part of the patient's limb. Better illustration of the natural history of this ill-defined pathology is needed to aid understanding and improve patient care.
Bello SOZ, Sarkar PK, 2010, SPONTANEOUS RUPTURE OF THE DIAPHRAGM: A DIAGNOSTIC ENIGMA, JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY, Vol: 140, Pages: 1440-1440, ISSN: 0022-5223
Bello S, Ferguson C, Wallis R, 2010, Improvements to the newborn bloodspot screening service are required to meet national standards, Journal of Medical Screening, Vol: 17, Pages: 114-120, ISSN: 0969-1413
<jats:sec><jats:title>Objective</jats:title><jats:p> The UK Newborn Screening Programme has standards to facilitate the early identification and treatment of five specific diseases to prevent lifelong impairment or death. This audit aimed to assess the newborn bloodspot screening programme in an inner London borough and to make recommendations for service improvement. </jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p> Data on babies registered with a general practitioner in the borough between April 2008 and March 2009 were obtained from the borough's health database and the laboratory result systems and compared with national screening standards. Interviews were conducted with the leads of each service providing components of newborn screening. </jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p> A total of 292 (8%) out of 3636 babies registered within the audit period had no result on the database (average 1 in 13). Regional reports revealed that over the previous three years the borough, like many others, had consistently failed to achieve the core standards for newborn bloodspot screening. Major areas of concern identified by this audit pertained to the quality of the bloodspot sample, response to requests for repeat samples, timely identification of untested babies and communication of results to parents. </jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p> The audit revealed that for the majority of children registered in the borough, screening was successfully carried out. However, gaps in the service meant that with current practice one affected child could be missed every seven years. Recommendations include staff training, frequent data reviews, and providing a coordinating officer to oversee the programme and follow up missing results. </jats:p></jats:sec>
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