Imperial College London
Alternate

DrStefanoCacciatore

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2137s.cacciatore

 
 
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Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Paglia:2015:10.1021/acs.jproteome.5b01020,
author = {Paglia, G and Stocchero, M and Cacciatore, S and Lai, S and Angel, P and Alam, MT and Keller, M and Ralser, M and Astarita, G},
doi = {10.1021/acs.jproteome.5b01020},
journal = {Journal of Proteome Research},
pages = {608--618},
title = {Unbiased Metabolomic Investigation of Alzheimer’s Disease Brain Points to Dysregulation of Mitochondrial Aspartate Metabolism},
url = {http://dx.doi.org/10.1021/acs.jproteome.5b01020},
volume = {15},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Alzheimer’s disease (AD) is the most common cause of adult dementia. Yet the complete set of molecular changes accompanying this inexorable, neurodegenerative disease remains elusive. Here we adopted an unbiased lipidomics and metabolomics approach to surveying frozen frontal cortex samples from clinically characterized AD patients (n = 21) and age-matched controls (n = 19), revealing marked molecular differences between them. Then, by means of metabolomic pathway analysis, we incorporated the novel molecular information into the known biochemical pathways and compared it with the results of a metabolomics meta-analysis of previously published AD research. We found six metabolic pathways of the central metabolism as well as glycerophospholipid metabolism predominantly altered in AD brains. Using targeted metabolomics approaches and MS imaging, we confirmed a marked dysregulation of mitochondrial aspartate metabolism. The altered metabolic pathways were further integrated with clinical data, showing various degrees of correlation with parameters of dementia and AD pathology. Our study highlights specific, altered biochemical pathways in the brains of individuals with AD compared with those of control subjects, emphasizing dysregulation of mitochondrial aspartate metabolism and supporting future venues of investigation.
AU  - Paglia,G
AU  - Stocchero,M
AU  - Cacciatore,S
AU  - Lai,S
AU  - Angel,P
AU  - Alam,MT
AU  - Keller,M
AU  - Ralser,M
AU  - Astarita,G
DO  - 10.1021/acs.jproteome.5b01020
EP  - 618
PY  - 2015///
SN  - 1535-3907
SP  - 608
TI  - Unbiased Metabolomic Investigation of Alzheimer’s Disease Brain Points to Dysregulation of Mitochondrial Aspartate Metabolism
T2  - Journal of Proteome Research
UR  - http://dx.doi.org/10.1021/acs.jproteome.5b01020
UR  - http://hdl.handle.net/10044/1/40685
VL  - 15
ER  -
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