16 results found
Dyar KA, Lutter D, Artati A, et al., 2018, Atlas of Circadian Metabolism Reveals System-wide Coordination and Communication between Clocks, CELL, Vol: 174, Pages: 1571-+, ISSN: 0092-8674
de Mateo S, Sassone-Corsi P, 2014, Regulation of spermatogenesis by small non-coding RNAs: Role of the germ granule, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, Vol: 29, Pages: 84-92, ISSN: 1084-9521
Eckel-Mahan KL, Patel VR, de Mateo S, et al., 2013, Reprogramming of the Circadian Clock by Nutritional Challenge, CELL, Vol: 155, Pages: 1464-1478, ISSN: 0092-8674
de Mateo S, Estanyol JM, Oliva R, 2013, Methods for the analysis of the sperm proteome., Methods Mol Biol, Vol: 927, Pages: 411-422
Proteomics is the study of the proteins of cells or tissues. Sperm proteomics aims at the identification of the proteins that compose the sperm cell and the study of their function. The recent developments in mass spectrometry (MS) have markedly increased the throughput for the identification and study of the sperm proteins. Catalogues of spermatozoal proteins in human and in model species are becoming available laying the groundwork for subsequent research, diagnostic applications, and the development of patient-specific treatments. A wide range of MS techniques is also rapidly becoming available for researchers. This chapter describes a methodological option to study the sperm cell using MS and provides a detailed protocol to identify the proteins extracted from a Percoll-purified human sperm population and separated by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) using LC-MS/MS.
Peruquetti RL, De Mateo S, Sassone-Corsi P, 2012, Circadian Proteins CLOCK and BMAL1 in the Chromatoid Body, a RNA Processing Granule of Male Germ Cells, PLOS ONE, Vol: 7, ISSN: 1932-6203
de Mateo S, Castillo J, Maria Estanyol J, et al., 2011, Proteomic characterization of the human sperm nucleus, PROTEOMICS, Vol: 11, Pages: 2714-2726, ISSN: 1615-9853
de Mateo S, Ramos L, van der Vlag J, et al., 2011, Improvement in chromatin maturity of human spermatozoa selected through density gradient centrifugation, INTERNATIONAL JOURNAL OF ANDROLOGY, Vol: 34, Pages: 256-267, ISSN: 0105-6263
Castillo J, Simon L, de Mateo S, et al., 2011, Protamine/DNA Ratios and DNA Damage in Native and Density Gradient Centrifuged Sperm From Infertile Patients, JOURNAL OF ANDROLOGY, Vol: 32, Pages: 324-332, ISSN: 0196-3635
de Mateo S, Gazquez C, Guimera M, et al., 2009, Protamine 2 precursors (Pre-P2), protamine 1 to protamine 2 ratio (P1/P2), and assisted reproduction outcome, FERTILITY AND STERILITY, Vol: 91, Pages: 715-722, ISSN: 0015-0282
Oliva R, de Mateo S, Estanyol JM, 2009, Sperm cell proteomics, PROTEOMICS, Vol: 9, Pages: 1004-1017, ISSN: 1615-9853
Gazquez C, Oriola J, de Mateo S, et al., 2008, A common protamine 1 promoter polymorphism (-190 c -> A) correlates with abnormal sperm morphology and increased protamine P1/P2 ratio in infertile patients, JOURNAL OF ANDROLOGY, Vol: 29, Pages: 540-548, ISSN: 0196-3635
Martinez-Heredia J, de Mateo S, Vidal-Taboada JM, et al., 2008, Identification of proteomic differences in asthenozoospermic sperm samples, HUMAN REPRODUCTION, Vol: 23, Pages: 783-791, ISSN: 0268-1161
de Mateo S, Martinez-Heredia J, Estanyol JM, et al., 2007, Marked correlations in protein expression identified by proteomic analysis of human spermatozoa, PROTEOMICS, Vol: 7, Pages: 4264-4277, ISSN: 1615-9853
Oliva R, Martínez-Heredia J, de Mateo S, et al., 2007, Proteomics of human spermatozoa, protamine content and assisted reproduction outcome., Soc Reprod Fertil Suppl, Vol: 65, Pages: 527-530, ISSN: 1747-3403
It is well known that alterations in the expression of the major sperm nuclear proteins (protamines) are related to infertility in man. In addition, other minor proteins extracted from human spermatozoa are being analysed by 2-dimensional polyacrylamide gel electrophoresis (2-D PAGE) and identified by MALDI-TOF MS analysis. The function of the identified proteins turns out to be energy production, transcription, protein synthesis, transport, folding and turnover, cell cycle, apoptosis and oxidative stress, signal transduction, cytoskeleton, flagella and cell movement, cell recognition, metabolism and unknown function. Many of the proteins identified using MALDI-TOF had not been yet been described as being expressed in human spermatozoa. Substantial differences have been detected in the levels of some of the newly identified human sperm proteins in the different groups of infertile patients. Present research efforts are targeting the potential correlations among changes in the proteomic composition, protamine content, DNA integrity and assisted reproduction outcome.
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