Imperial College London
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Emeritus ProfessorStephenFranks

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Emeritus Professor of Reproductive Endocrinology
 
 
 
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Contact

 

+44 (0)20 7594 2109s.franks Website

 
 
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Assistant

 

Miss Kiran Dosanjh +44 (0)20 7594 4217

 
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Location

 

5009Institute of Reproductive and Developmental BiologyHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Makrinou:2019:10.1016/j.mce.2019.110611,
author = {Makrinou, E and Drong, AW and Christopoulos, G and Lerner, A and Chapa-Chorda, I and Karaderi, T and Lavery, S and Hardy, K and Lindgren, CM and Franks, S},
doi = {10.1016/j.mce.2019.110611},
journal = {Molecular and Cellular Endocrinology},
pages = {1--11},
title = {Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS).},
url = {http://dx.doi.org/10.1016/j.mce.2019.110611},
volume = {500},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Polycystic Ovary Syndrome (PCOS) is the most common endocrine disorder amongst women of reproductive age, whose aetiology remains unclear. To improve our understanding of the molecular mechanisms underlying the disease, we conducted a genome-wide DNA methylation profiling in granulosa lutein cells collected from 16 women suffering from PCOS, in comparison to 16 healthy controls. Samples were collected by follicular aspiration during routine egg collection for IVF treatment. Study groups were matched for age and BMI, did not suffer from other disease and were not taking confounding medication. Comparing women with polycystic versus normal ovarian morphology, after correcting for multiple comparisons, we identified 106 differentially methylated CpG sites with p-values <5.8×10-8 that were associated with 88 genes, several of which, are known to relate either to PCOS or to ovarian function. Replication and validation of the experiment was done using pyrosequencing to analyse six of the identified differentially methylated sites. Pathway analysis indicated potential disruption in canonical pathways and gene networks that are, amongst other, associated with cancer, cardiogenesis, Hedgehog signalling and immune response. In conclusion, these novel findings indicate that women with PCOS display epigenetic changes in ovarian granulosa cells that may be associated with the heterogeneity of the disorder.
AU  - Makrinou,E
AU  - Drong,AW
AU  - Christopoulos,G
AU  - Lerner,A
AU  - Chapa-Chorda,I
AU  - Karaderi,T
AU  - Lavery,S
AU  - Hardy,K
AU  - Lindgren,CM
AU  - Franks,S
DO  - 10.1016/j.mce.2019.110611
EP  - 11
PY  - 2019///
SN  - 0303-7207
SP  - 1
TI  - Genome-wide methylation profiling in granulosa lutein cells of women with polycystic ovary syndrome (PCOS).
T2  - Molecular and Cellular Endocrinology
UR  - http://dx.doi.org/10.1016/j.mce.2019.110611
UR  - https://www.ncbi.nlm.nih.gov/pubmed/31600550
UR  - https://www.sciencedirect.com/science/article/pii/S0303720719303132
UR  - http://hdl.handle.net/10044/1/74400
VL  - 500
ER  -
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