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@article{Kristensen:2022:clinem/dgac272,
author = {Kristensen, SG and Kumar, A and Mamsen, LS and Kalra, B and Pors, SE and Botkjaer, JA and Macklon, KT and Fedder, J and Ernst, E and Hardy, K and Franks, S and Andersen, CY},
doi = {clinem/dgac272},
journal = {Journal of Clinical Endocrinology and Metabolism},
pages = {E3374--E3383},
title = {Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries},
url = {http://dx.doi.org/10.1210/clinem/dgac272},
volume = {107},
year = {2022}
}

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TY  - JOUR
AB  - ContextThe oocyte-secreted factors growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) play essential roles in follicle development and oocyte maturation, and aberrant regulation might contribute to the pathogenesis of polycystic ovary syndrome.ObjectiveAre there measurable differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in small antral follicle fluids from women with and without polycystic ovaries (PCO)?Design and SettingFollicle fluids (n=356) were collected from 4- to 11-mm follicles in unstimulated ovaries of 87 women undergoing ovarian tissue cryopreservation for fertility preservation.PatientsTwenty-seven women with PCO were identified and 60 women without PCO-like characteristics (non-PCO women) were matched according to age and follicle size.Main outcome measuresIntrafollicular concentrations of GDF9, BMP15, GDF9/BMP15 heterodimer, anti-Mullerian hormone (AMH), inhibin-A and -B, total inhibin, activin-B and -AB, and follistatin were measured using enzyme-linked immunosorbent assays.ResultsThe detectability of GDF9, BMP15, and the GDF9/BMP15 heterodimer were 100%, 94.4%, and 91.5%, respectively, and concentrations were significantly negatively correlated with increasing follicle size (P<0.0001). GDF9 was significantly higher in women with PCO (PCO: 4230±189 pg/mL [mean±SEM], n=188; non-PCO: 3498±199 pg/mL, n=168; P<0.03), whereas BMP15 was lower in women with PCO (PCO: 431±40 pg/mL, n=125; non-PCO: 573±55 pg/mL, n=109; P=0.10), leading to a significantly higher GDF9:BMP15 ratio in women with PCO (P<0.01). Significant positive associations between BMP15 and AMH, activins, and inhibins in non-PCO women switched to negative associations in women with PCO.ConclusionsIntrafollicular concentrations of GDF9 and BMP15 varied inversely in women with PCO reflecting an aberrant endocrine environment. An increased GDF9:BMP15 ratio may be a new
AU  - Kristensen,SG
AU  - Kumar,A
AU  - Mamsen,LS
AU  - Kalra,B
AU  - Pors,SE
AU  - Botkjaer,JA
AU  - Macklon,KT
AU  - Fedder,J
AU  - Ernst,E
AU  - Hardy,K
AU  - Franks,S
AU  - Andersen,CY
DO  - clinem/dgac272
EP  - 3383
PY  - 2022///
SN  - 0021-972X
SP  - 3374
TI  - Intrafollicular concentrations of the oocyte-secreted factors GDF9 and BMP15 vary inversely in polycystic ovaries
T2  - Journal of Clinical Endocrinology and Metabolism
UR  - http://dx.doi.org/10.1210/clinem/dgac272
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000798062000001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/jcem/article/107/8/e3374/6576537
UR  - http://hdl.handle.net/10044/1/98570
VL  - 107
ER  -

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