Imperial College London
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ProfessorStuartHaslam

Faculty of Natural Sciences, Department of Life Sciences

Professor in Structural Glycobiology
 
 
 
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Contact

 

+44 (0)20 7594 5222s.haslam

 
 
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Location

 

101ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Silver:2020:10.1016/j.celrep.2020.01.056,
author = {Silver, ZA and Antonopoulos, A and Haslam, SM and Dell, A and Dickinson, GM and Seaman, MS and Desrosiers, RC},
doi = {10.1016/j.celrep.2020.01.056},
journal = {Cell Reports},
pages = {1862--1869.e4},
title = {Discovery of O-linked carbohydrate on HIV-1 envelope and its role in shielding against one category of broadly neutralizing antibodies},
url = {http://dx.doi.org/10.1016/j.celrep.2020.01.056},
volume = {30},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Approximately 50% of the mass of the Envelope (Env) glycoprotein surface subunit (gp120) of human immunodeficiency virus type 1 (HIV-1) is composed of N-linked carbohydrate. Until now, the dogma has been that HIV-1 lacks O-linked carbohydrate on Env. Here we show that a subset of patient-derived HIV-1 isolates contain O-linked carbohydrate on the variable 1 (V1) domain of Env gp120. We demonstrate the presence of this O-glycosylation both on virions and on gp120 expressed as a secreted protein. Further, we establish that these O-linked glycans can confer a more than 1,000-fold decrease in neutralization sensitivity (IC50) to V3-glycan broadly neutralizing antibodies. These findings uncover a structural modification to the HIV-1 Env and suggest a functional role in promoting viral escape from one category of broadly neutralizing antibodies.
AU  - Silver,ZA
AU  - Antonopoulos,A
AU  - Haslam,SM
AU  - Dell,A
AU  - Dickinson,GM
AU  - Seaman,MS
AU  - Desrosiers,RC
DO  - 10.1016/j.celrep.2020.01.056
EP  - 1869
PY  - 2020///
SN  - 2211-1247
SP  - 1862
TI  - Discovery of O-linked carbohydrate on HIV-1 envelope and its role in shielding against one category of broadly neutralizing antibodies
T2  - Cell Reports
UR  - http://dx.doi.org/10.1016/j.celrep.2020.01.056
UR  - https://www.cell.com/cell-reports/fulltext/S2211-1247(20)30081-4?_returnURL=https%3A%2F%2Flinkinghub.elsevier.com%2Fretrieve%2Fpii%2FS2211124720300814%3Fshowall%3Dtrue
UR  - http://hdl.handle.net/10044/1/76893
VL  - 30
ER  -
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