Imperial College London
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ProfessorStuartHaslam

Faculty of Natural Sciences, Department of Life Sciences

Professor in Structural Glycobiology
 
 
 
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Contact

 

+44 (0)20 7594 5222s.haslam

 
 
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Location

 

101ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Baksmeier:2021:10.1111/imm.13341,
author = {Baksmeier, C and Blundell, P and Steckel, J and Schultz, V and Gu, Q and Da, Silva Filipe A and Kohl, A and Linnington, C and Lu, D and Dell, A and Haslam, S and Wang, J and Czajkowsky, D and Goebels, N and Pleass, RJ},
doi = {10.1111/imm.13341},
journal = {Immunology},
pages = {90--105},
title = {Modified recombinant human IgG1-Fc is superior to natural IVIG at inhibiting immune-mediated demyelination.},
url = {http://dx.doi.org/10.1111/imm.13341},
volume = {464},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Intravenous immunoglobulin (IVIG) is an established treatment for numerous autoimmune conditions. Although Fc fragments derived from IVIG have shown efficacy in controlling immune thrombocytopenia (ITP) in children, the mechanisms of action are unclear and controversial. The aim of this study is to dissect IVIG effector mechanisms using further adapted Fc fragments on demyelination in an ex vivo model of the central nervous system (CNS)-immune interface. Using organotypic cerebellar slice cultures (OSC) from transgenic mice we induced extensive immune-mediated demyelination and oligodendrocyte loss with an antibody specific for myelin oligodendrocyte glycoprotein (MOG) and complement. Protective effects of adapted Fc fragments were assessed by live imaging of GFP expression, immunohistochemistry and confocal microscopy. Cysteine and glycan adapted Fc fragments protected OSC from demyelination in a dose-dependent manner where equimolar concentrations of either IVIG or control Fc were ineffective. The protective effects of the adapted Fc fragments are partly attributed to interference with complement-mediated oligodendroglia damage. Transcriptome analysis ruled out signatures associated with inflammatory or innate immune responses. Taken together our findings show that recombinant biomimetics can be made that are at least two hundred-fold more effective than IVIG in controlling demyelination by anti-MOG antibodies.
AU  - Baksmeier,C
AU  - Blundell,P
AU  - Steckel,J
AU  - Schultz,V
AU  - Gu,Q
AU  - Da,Silva Filipe A
AU  - Kohl,A
AU  - Linnington,C
AU  - Lu,D
AU  - Dell,A
AU  - Haslam,S
AU  - Wang,J
AU  - Czajkowsky,D
AU  - Goebels,N
AU  - Pleass,RJ
DO  - 10.1111/imm.13341
EP  - 105
PY  - 2021///
SN  - 0019-2805
SP  - 90
TI  - Modified recombinant human IgG1-Fc is superior to natural IVIG at inhibiting immune-mediated demyelination.
T2  - Immunology
UR  - http://dx.doi.org/10.1111/imm.13341
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33880776
UR  - https://onlinelibrary.wiley.com/doi/10.1111/imm.13341
UR  - http://hdl.handle.net/10044/1/88345
VL  - 464
ER  -
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