Imperial College London
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ProfessorStuartHaslam

Faculty of Natural Sciences, Department of Life Sciences

Professor in Structural Glycobiology
 
 
 
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Contact

 

+44 (0)20 7594 5222s.haslam

 
 
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Location

 

101ASir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Ezeabikwa:2021:glycob/cwab083,
author = {Ezeabikwa, B and Mondal, N and Antonopoulos, A and Haslam, SM and Matsumoto, Y and Martin-Caraballo, M and Lehoux, S and Mandalasi, M and Ishaque, A and Heimburg-Molinaro, J and Cummings, RD and Nyame, AK},
doi = {glycob/cwab083},
journal = {Glycobiology},
pages = {1444--1463},
title = {Major differences in glycosylation and Fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.},
url = {http://dx.doi.org/10.1093/glycob/cwab083},
volume = {31},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Bladder cancer is the ninth most frequently diagnosed cancer worldwide, and there is a need to develop new biomarkers for staging and prognosis of this disease. Here we report that cell lines derived from low-grade and high-grade bladder cancers exhibit major differences in expression of glycans in surface glycoproteins. We analyzed protein glycosylation in three low-grade bladder cancer cell lines RT4 (grade-1-2), 5637 (grade-2), and SW780 (grade-1), and three high-grade bladder cancer cell lines J82COT (grade-3), T24 (grade-3), and TCCSUP (grade-4), with primary bladder epithelial cells, A/T/N, serving as a normal bladder cell control. Using a variety of approaches including flow cytometry, immunofluorescence, glycomics, and gene expression analysis, we observed that the low-grade bladder cancer cell lines RT4, 5637, and SW780 express high levels of the fucosylated Lewis x (Lex) antigen (CD15) (Galβ1-4(Fucα1-3) GlcNAcβ1-R), while normal bladder epithelial A/T/N cells lack Lex expression. T24 and TCCSUP cells also lack Lex, whereas J82COT cells express low levels of Lex. Glycomics analyses revealed other major differences in fucosylation and sialylation of N-glycans between these cell types. O-glycans are highly differentiated, as RT4 cells synthesize core 2-based O-glycans that are lacking in the T24 cells. These differences in glycan expression correlated with differences in RNA expression levels of their cognate glycosyltransferases, including α1-3/4-fucosyltransferase genes. These major differences in glycan structures and gene expression profiles between low- and high-grade bladder cancer cells suggest that glycans and glycosyltransferases are candidate biomarkers for grading bladder cancers.
AU  - Ezeabikwa,B
AU  - Mondal,N
AU  - Antonopoulos,A
AU  - Haslam,SM
AU  - Matsumoto,Y
AU  - Martin-Caraballo,M
AU  - Lehoux,S
AU  - Mandalasi,M
AU  - Ishaque,A
AU  - Heimburg-Molinaro,J
AU  - Cummings,RD
AU  - Nyame,AK
DO  - glycob/cwab083
EP  - 1463
PY  - 2021///
SN  - 0959-6658
SP  - 1444
TI  - Major differences in glycosylation and Fucosyltransferase expression in low-grade versus high-grade bladder cancer cell lines.
T2  - Glycobiology
UR  - http://dx.doi.org/10.1093/glycob/cwab083
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34350945
UR  - https://academic.oup.com/glycob/advance-article/doi/10.1093/glycob/cwab083/6338188
UR  - http://hdl.handle.net/10044/1/90985
VL  - 31
ER  -
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