Imperial College London

ProfessorStuartHaslam

Faculty of Natural SciencesDepartment of Life Sciences

Professor in Structural Glycobiology
 
 
 
//

Contact

 

+44 (0)20 7594 5222s.haslam

 
 
//

Location

 

101ASir Ernst Chain BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Cao:2021:10.12688/wellcomeopenres.16409.1,
author = {Cao, H and Bagchi, A and Tampakis, D and Laidvee, I and Williams, M and Minter, B and Wright, S and Antonopoulos, A and Haslam, SM and Barker, RN and Vickers, MA},
doi = {10.12688/wellcomeopenres.16409.1},
journal = {Wellcome Open Research},
pages = {1--14},
title = {Human erythrocyte surface fucose expression increases with age and hyperglycemia},
url = {http://dx.doi.org/10.12688/wellcomeopenres.16409.1},
volume = {6},
year = {2021}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Background: Reactive oxygen species and other free radicals, together with glucose and its metabolites are believed to play important roles in the aging process. The carbohydrate components of glycosylated proteins are important in mediating cell-cell interactions and a role has been suggested for them in the aging process. Erythrocytes are critical cells in the human body, heavily glycosylated and relatively easily available and so are good candidates to yield insights into how patterns of glycosylation change with age and disease. It has been claimed, based on a periodic acid Schiff assay, that human aging is associated with a decline of erythrocyte surface sialic acids. Plant lectins allow for more specific assays for glycans, including determining the linkage of sialic acids and analysis of single cells by flow cytometry.Methods: Plant lectins, including Maackia amurensis lectin II (MAL), binding to α-2,3 linked sialic acids and Sambucus nigra (SNA), α-2,6 sialic acids, were used in flow cytometry and western blot of erythrocyte surface membrane. N-glycomics mass spectrometry determines glycan structures. Donors varying in age and hyperglycemia, as indicated by HbA1c were analysed.Results: Erythrocyte surface sialic acids have no significant associations with donor age. A combination of storage and cellular aging produces a specific loss of α-2,6 sialic acids. By contrast, erythrocyte surface terminal fucoses increase significantly with donor age. In order to determine which aspects of aging are important in determining this change, we investigated whether this novel human aging biomarker is associated with higher plasma glucose values, assessed by glycated hemoglobin (HbA1c) and reactive oxygen species (ROS) generation. Fucose levels were associated with HbA1c levels, but not ROS generation.Conclusion: Our study identifies novel glycan-based biomarkers for human aging and disease. The simplicity of lectin-based assays provide an attractive cel
AU - Cao,H
AU - Bagchi,A
AU - Tampakis,D
AU - Laidvee,I
AU - Williams,M
AU - Minter,B
AU - Wright,S
AU - Antonopoulos,A
AU - Haslam,SM
AU - Barker,RN
AU - Vickers,MA
DO - 10.12688/wellcomeopenres.16409.1
EP - 14
PY - 2021///
SN - 2398-502X
SP - 1
TI - Human erythrocyte surface fucose expression increases with age and hyperglycemia
T2 - Wellcome Open Research
UR - http://dx.doi.org/10.12688/wellcomeopenres.16409.1
UR - https://wellcomeopenresearch.org/articles/6-28/v1
UR - http://hdl.handle.net/10044/1/92359
VL - 6
ER -