Imperial College London
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DrSarahHedberg

Faculty of Engineering, Department of Chemical Engineering

 
 
 
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Contact

 

+44 (0)20 7594 6227s.hedberg13

 
 
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Location

 

C410Roderic Hill BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Hou:2012:10.1186/2046-1682-5-8,
author = {Hou, Y and Hedberg, S and Schneider, IC},
doi = {10.1186/2046-1682-5-8},
journal = {BMC Biophysics},
title = {Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation},
url = {http://dx.doi.org/10.1186/2046-1682-5-8},
volume = {5},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title>          <jats:sec>            <jats:title>Background</jats:title>            <jats:p>Cell migration plays an essential role in many biological processes, such as cancer metastasis, wound healing and immune response. Cell migration is mediated through protrusion and focal adhesion (FA) assembly, maturation and disassembly. Epidermal growth factor (EGF) is known to enhance migration rate in many cell types; however it is not known how FA maturation, FA dynamics and protrusion dynamics are regulated during EGF-induced migration. Here we use total internal reflection fluorescence (TIRF) microscopy and image analysis to quantify FA properties and protrusion dynamics under different doses of EGF stimulation.</jats:p>          </jats:sec>          <jats:sec>            <jats:title>Results</jats:title>            <jats:p>EGF was found to broaden the distribution of cell migration rates, generating more fast and slow cells. Furthermore, groups based on EGF stimulation condition or cell migration speed were marked by characteristic signatures. When data was binned based on EGF stimulation conditions, FA intensity and FA number per cell showed the largest difference among stimulation groups. FA intensity decreased with increasing EGF concentration and FA number per cell was highest under intermediate stimulation conditions. No difference in protrusion behavior was observed. However, when data was binned based on cell migration speed, FA intensity and not FA number per cell showed the largest difference among groups. FA intensity was lower for fast migrating cells. Additionally, waves of protrusion tended to correlate with fast migrating cells.</jats:p>          </jats:sec>          <jats:sec>            <jats:title>Conclusions</jats:title>            <jats:p>Only a portion of the FA properties and protrusion dynamics that correlate with
AU  - Hou,Y
AU  - Hedberg,S
AU  - Schneider,IC
DO  - 10.1186/2046-1682-5-8
PY  - 2012///
TI  - Differences in adhesion and protrusion properties correlate with differences in migration speed under EGF stimulation
T2  - BMC Biophysics
UR  - http://dx.doi.org/10.1186/2046-1682-5-8
VL  - 5
ER  -
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