Imperial College London
Alternate

Prof Steve Matthews

Faculty of Natural Sciences, Department of Life Sciences

Professor of Chemical and Structural Biology
 
 
 
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Contact

 

+44 (0)20 7594 5315s.j.matthews Website

 
 
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Location

 

602Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sewell:2020:10.1038/s41598-020-64135-9,
author = {Sewell, L and Stylianou, F and Xu, Y and Taylor, J and Sefer, L and Matthews, S},
doi = {10.1038/s41598-020-64135-9},
journal = {Scientific Reports},
title = {NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA},
url = {http://dx.doi.org/10.1038/s41598-020-64135-9},
volume = {10},
year = {2020}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The biofilms of Enterobacteriaceae are fortified by assembly of curli amyloid fibres on the cell surface. Curli not only provides structural reinforcement, but also facilitates surface adhesion. To prevent toxic intracellular accumulation of amyloid precipitate, secretion of the major curli subunit, CsgA, is tightly regulated. In this work, we have employed solution state NMR spectroscopy to characterise the structural ensemble of the pre-fibrillar state of CsgA within the bacterial periplasm, and upon recruitment to the curli pore, CsgG, and the secretion chaperone, CsgE. We show that the N-terminal targeting sequence (N) of CsgA binds specifically to CsgG and that its subsequent sequestration induces a marked transition in the conformational ensemble, which is coupled to a preference for CsgE binding. These observations lead us to suggest a sequential model for binding and structural rearrangement of CsgA at the periplasmic face of the secretion machinery.
AU  - Sewell,L
AU  - Stylianou,F
AU  - Xu,Y
AU  - Taylor,J
AU  - Sefer,L
AU  - Matthews,S
DO  - 10.1038/s41598-020-64135-9
PY  - 2020///
SN  - 2045-2322
TI  - NMR insights into the pre-amyloid ensemble and secretion targeting of the curli subunit CsgA
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/s41598-020-64135-9
UR  - http://hdl.handle.net/10044/1/79248
VL  - 10
ER  -
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