Imperial College London
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DrSarahJoseph

Faculty of Medicine, Department of Infectious Disease

Clinical Immunologist
 
 
 
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Contact

 

s.joseph

 
 
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Location

 

457Medical SchoolSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gombe:2022:10.1371/journal.pone.0275927,
author = {Gombe, B and Streatfield, C and Leal, L and Opio, S and Joseph, S and Weber, J and Hare, J and Kaleebu, P and Serwanga, J},
doi = {10.1371/journal.pone.0275927},
journal = {PLoS One},
title = {Optimization and validation of an ELISA assay for the determination of antibody responses to CN54gp140 and AIDSVAX BE for use in the Phase IIb PrEPVacc vaccine trial},
url = {http://dx.doi.org/10.1371/journal.pone.0275927},
volume = {17},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - PrEPVacc is an international, multi-centre, double-blind vaccine study comparing experimental combination vaccine regimens including DNA/AIDSVAX BE and DNA/CN54gp140 with placebo control. Simultaneously, daily oral PrEP is compared for efficacy against daily Truvada in the context of the current PrEP availability situation at the study sites. An important clinical trial outcome is the accurate measurement of in vivo antibody titer induced through vaccination. Here we report the validation of two ELISAs for CN54gp140 and AIDSVAX BE at Uganda Virus Research Institute that demonstrates precision, specificity, and robustness for assessing the reciprocal antibody end point titer in human serum. This is a critical endpoint for determining whether vaccination can provide any protection against HIV in populations at risk of acquiring HIV.
AU  - Gombe,B
AU  - Streatfield,C
AU  - Leal,L
AU  - Opio,S
AU  - Joseph,S
AU  - Weber,J
AU  - Hare,J
AU  - Kaleebu,P
AU  - Serwanga,J
DO  - 10.1371/journal.pone.0275927
PY  - 2022///
SN  - 1932-6203
TI  - Optimization and validation of an ELISA assay for the determination of antibody responses to CN54gp140 and AIDSVAX BE for use in the Phase IIb PrEPVacc vaccine trial
T2  - PLoS One
UR  - http://dx.doi.org/10.1371/journal.pone.0275927
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36327320
UR  - http://hdl.handle.net/10044/1/101387
VL  - 17
ER  -
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